Aldosterone regulates Na,K-ATPase and increases lung edema clearance in rats

Walter G. Olivera, David E. Ciccolella, Nora Barquin, Karen M. Ridge, David H. Rutschman, Donovan B. Yeates, Jacob I. Sznajder*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


Aldosterone increases the Na,K-ATPase function in renal cells involved in active Na+ transport. Because the alveolar type 2 (AT2) cells participate in active Na+ transport, we studied whether aldosterone regulates the Na,K- ATPase in rat AT2 cells and whether aldosterone delivered by aerosols to spontaneously breathing rats affects edema clearance in a model of isolated- perfused lungs. The AT2 cells treated with aldosterone had increased Na,K- ATPase β1-subunit mRNA and protein, which was associated with a 4-fold increase in the Na,K-ATPase hydrolytic activity and the ouabain-sensitive 86Rb+ uptake. In physiologic experiments, 24 h after aldosterone was delivered by aerosols to the rat air spaces, the active Na+ transport and lung edema clearance increased by ~ 53% as compared with control rats and rats in which saline aerosols were delivered. The data suggest that increased active Na+ transport and lung edema clearance induced by aldosterone is probably due to Na,K-ATPase regulation in alveolar epithelial cells. Conceivably, aldosterone may be used as a strategy to increase lung edema clearance.

Original languageEnglish (US)
Pages (from-to)567-573
Number of pages7
JournalAmerican journal of respiratory and critical care medicine
Issue number2 I
StatePublished - 2000

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine


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