Alfaxalone-xylazine anesthesia in laboratory mice (Mus musculus)

Rebecca L. Erickson, Caroline E. Blevins, Cecilia De Souza Dyer, James O. Marx*

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Since its recent reformulation, alfaxalone has gained popularity as an injectable veterinary anesthetic, including promising studies demonstrating the use of alfaxalone-xylazine for anesthesia in mice. Here we sought to expand these studies by testing additional dose ranges, elaborating on physiologic monitoring, testing sex- and strain-associated differences, and evaluating efficacy during actual surgical conditions. C57BL/6J mice showed significant sex-associated differences in anesthetic sensitivity, with males requiring higher doses of alfaxalone (80-120 mg/kg IP alfaxalone with 10 mg/kg IP xylazine) than females (40-80 mg/kg IP alfaxalone with 10 mg/kg IP xylazine) to achieve a surgical plane of anesthesia. In addition, female outbred CD1 mice were less sensitive to alfaxalone than female inbred C57BL/6J mice. When used during actual surgery, alfaxalone- xylazine administered intraperitoneally provided adequate anesthesia for a model of orthopedic surgery, whereas the same anesthetic regimen during laparotomy resulted in unacceptably high mortality; survival during laparotomy increased when drugs were administered subcutaneously. These results indicate that alfaxalone-xylazine may be a viable option for injectable surgical anesthesia in mice, although strain- and sex-associated differences and alternative routes of administration should be considered when optimizing the anesthetic regimen for specific experimental conditions.

Original languageEnglish (US)
Pages (from-to)30-39
Number of pages10
JournalJournal of the American Association for Laboratory Animal Science
Volume58
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

xylazine
Mus musculus
anesthesia
anesthetics
mice
laparotomy
gender
surgery
orthopedics
dosage
testing
drugs
monitoring

Keywords

  • HR
  • Heart rate
  • LORR
  • LPWR
  • Loss of pedal withdrawal reflex
  • Loss of righting reflex
  • PWR
  • Pedal withdrawal reflex
  • RR
  • Respiratory rate

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Erickson, Rebecca L. ; Blevins, Caroline E. ; De Souza Dyer, Cecilia ; Marx, James O. / Alfaxalone-xylazine anesthesia in laboratory mice (Mus musculus). In: Journal of the American Association for Laboratory Animal Science. 2019 ; Vol. 58, No. 1. pp. 30-39.
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Alfaxalone-xylazine anesthesia in laboratory mice (Mus musculus). / Erickson, Rebecca L.; Blevins, Caroline E.; De Souza Dyer, Cecilia; Marx, James O.

In: Journal of the American Association for Laboratory Animal Science, Vol. 58, No. 1, 01.01.2019, p. 30-39.

Research output: Contribution to journalArticle

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