Alteration of nuclear lamin organization inhibits RNA polymerase II-dependent transcription

Timothy P. Spann, Anne E. Goldman, Chen Wang, Sui Huang, Robert D. Goldman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

214 Scopus citations

Abstract

Regulation of gene activity is mediated by alterations in chromatin organization. In addition, chromatin organization may be governed in part by interactions with structural components of the nucleus. The nuclear lamins comprise the lamina and a variety of nucleoplasmic assemblies that together are major structural components of the nucleus. Furthermore, lamins and lamin-associated proteins have been reported to bind chromatin. These observations suggest that the nuclear lamins may be involved in the regulation of gene activity. In this report, we test this possibility by disrupting the normal organization of nuclear lamins with a dominant negative lamin mutant lacking the NH2-terminal domain. We find that this disruption inhibits RNA polymerase II activity in both mammalian cells and transcriptionally active embryonic nuclei from Xenopus laevis. The inhibition appears to be specific for polymerase II as disruption of lamin organization does not detectably inhibit RNA polymerases I and III. Furthermore, immunofluorescence observations indicate that this selective inhibition of polymerase II-dependent transcription involves the TATA binding protein, a component of the basal transcription factor TFIID.

Original languageEnglish (US)
Pages (from-to)603-608
Number of pages6
JournalJournal of Cell Biology
Volume156
Issue number4
DOIs
StatePublished - Feb 18 2002

Keywords

  • Lamins
  • RNA polymerase II
  • TBP
  • TFIID
  • Transcription

ASJC Scopus subject areas

  • Cell Biology

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