Alteration of the action potential of tissue cultured neuronal cells by growth in the presence of a polyunsaturated fatty acid

William R. Saum*, Richard McGee, Jeffrey Love

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The effects of alterations in membrane phospholipid fatty acid composition on the excitability of neuroblastoma × glioma hybrid cells, clone NG108-15, were examined using intracellular recording techniques. Cells were grown in the presence of arachidonate (20:4) added to the culture medium as a complex with bovine serum albumin. Exposure of the cells to 20:4 for 3-21 days produced a 40% decrease in the maximum rate of rise of the action potential (dV/dt) with a small change in its amplitude. The resting membrane potential and passive properties of the cells were unaffected. An effect of 20:4 was not observed until 24 hr after treatment and increased over the next 2 days. The phospholipid content of 20:4 and its metabolite 22:4 increased from 6.9% to 25.3% of total fatty acids during approximately the same time span. It is concluded that the action potential dV/dt can be altered by changes in membrane lipid composition.

Original languageEnglish (US)
Pages (from-to)319-324
Number of pages6
JournalCellular and Molecular Neurobiology
Volume1
Issue number3
DOIs
StatePublished - Sep 1 1981

Keywords

  • action potential
  • excitable membranes
  • membrane lipids
  • neuroblastoma cells

ASJC Scopus subject areas

  • Neuroscience(all)
  • Genetics
  • Clinical Biochemistry
  • Cell Biology

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