Abstract
The effects of alterations in membrane phospholipid fatty acid composition on the excitability of neuroblastoma × glioma hybrid cells, clone NG108-15, were examined using intracellular recording techniques. Cells were grown in the presence of arachidonate (20:4) added to the culture medium as a complex with bovine serum albumin. Exposure of the cells to 20:4 for 3-21 days produced a 40% decrease in the maximum rate of rise of the action potential (dV/dt) with a small change in its amplitude. The resting membrane potential and passive properties of the cells were unaffected. An effect of 20:4 was not observed until 24 hr after treatment and increased over the next 2 days. The phospholipid content of 20:4 and its metabolite 22:4 increased from 6.9% to 25.3% of total fatty acids during approximately the same time span. It is concluded that the action potential dV/dt can be altered by changes in membrane lipid composition.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 319-324 |
| Number of pages | 6 |
| Journal | Cellular and Molecular Neurobiology |
| Volume | 1 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 1 1981 |
Keywords
- action potential
- excitable membranes
- membrane lipids
- neuroblastoma cells
ASJC Scopus subject areas
- Neuroscience(all)
- Genetics
- Clinical Biochemistry
- Cell Biology