TY - JOUR
T1 - Alterations in c-abl gene methylation in cells transformed by phagocyte-generated oxidants
AU - Weitzman, Sigmund A.
AU - Lee, Robert M.
AU - Ouellette, Andre J.
N1 - Funding Information:
Supported by American Cancer Society Grant CD-190 and USPHS Grant CA-43906 and the Shriners Hospitals for Crippled Children. We thank Susan Bruno for secretarial support, Patrick Turk, and Dana Frederick for technical assistance and Drs. Thomas Stossel, Rex Chisholm and Ronald A. Malt for helpful discussions.
PY - 1989/1/16
Y1 - 1989/1/16
N2 - DNA from 10T1/2 cells transformed by activated neutrophils was analyzed for restriction length polymorphisms (RFLPs) in cellular homologues of retroviral oncogenes, and consistent RFLPs were found in MspI sites of the c-abl gene of all PMN-transformed cell lines. MspI digests probed with c-myc, v-Ki-ras, v-Ha-ras or v-mos showed no RFLPs, and none were observed in EcoRI, PstI, HindIII, BamHI, SmaI, Sau3a, MboI, HhaI, or TaqI digests probed with v-abl. Analysis of HpaII digests supports the conclusion that c-abl RFLPs result from differential methylation of the CCGG HpaII/MspI recognition sequence. MspI RFLPs in the c-abl gene may provide markers for oxidant-related genetic injury.
AB - DNA from 10T1/2 cells transformed by activated neutrophils was analyzed for restriction length polymorphisms (RFLPs) in cellular homologues of retroviral oncogenes, and consistent RFLPs were found in MspI sites of the c-abl gene of all PMN-transformed cell lines. MspI digests probed with c-myc, v-Ki-ras, v-Ha-ras or v-mos showed no RFLPs, and none were observed in EcoRI, PstI, HindIII, BamHI, SmaI, Sau3a, MboI, HhaI, or TaqI digests probed with v-abl. Analysis of HpaII digests supports the conclusion that c-abl RFLPs result from differential methylation of the CCGG HpaII/MspI recognition sequence. MspI RFLPs in the c-abl gene may provide markers for oxidant-related genetic injury.
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U2 - 10.1016/S0006-291X(89)80171-8
DO - 10.1016/S0006-291X(89)80171-8
M3 - Article
C2 - 2563223
AN - SCOPUS:0024579548
SN - 0006-291X
VL - 158
SP - 24
EP - 30
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -