Alterations in c-abl gene methylation in cells transformed by phagocyte-generated oxidants

Sigmund A. Weitzman*, Robert M. Lee, Andre J. Ouellette

*Corresponding author for this work

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

DNA from 10T1/2 cells transformed by activated neutrophils was analyzed for restriction length polymorphisms (RFLPs) in cellular homologues of retroviral oncogenes, and consistent RFLPs were found in MspI sites of the c-abl gene of all PMN-transformed cell lines. MspI digests probed with c-myc, v-Ki-ras, v-Ha-ras or v-mos showed no RFLPs, and none were observed in EcoRI, PstI, HindIII, BamHI, SmaI, Sau3a, MboI, HhaI, or TaqI digests probed with v-abl. Analysis of HpaII digests supports the conclusion that c-abl RFLPs result from differential methylation of the CCGG HpaII/MspI recognition sequence. MspI RFLPs in the c-abl gene may provide markers for oxidant-related genetic injury.

Original languageEnglish (US)
Pages (from-to)24-30
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume158
Issue number1
DOIs
StatePublished - Jan 16 1989

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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