Alterations in c-abl gene methylation in cells transformed by phagocyte-generated oxidants

Sigmund A. Weitzman; Robert M. Lee; Andre J. Ouellette

doi:10.1016/S0006-291X(89)80171-8

Alterations in c-abl gene methylation in cells transformed by phagocyte-generated oxidants

Sigmund A. Weitzman^*, Robert M. Lee, Andre J. Ouellette

^*Corresponding author for this work

Medicine, Hematology Oncology Division

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

DNA from 10T1/2 cells transformed by activated neutrophils was analyzed for restriction length polymorphisms (RFLPs) in cellular homologues of retroviral oncogenes, and consistent RFLPs were found in MspI sites of the c-abl gene of all PMN-transformed cell lines. MspI digests probed with c-myc, v-Ki-ras, v-Ha-ras or v-mos showed no RFLPs, and none were observed in EcoRI, PstI, HindIII, BamHI, SmaI, Sau3a, MboI, HhaI, or TaqI digests probed with v-abl. Analysis of HpaII digests supports the conclusion that c-abl RFLPs result from differential methylation of the CCGG HpaII/MspI recognition sequence. MspI RFLPs in the c-abl gene may provide markers for oxidant-related genetic injury.

Original language	English (US)
Pages (from-to)	24-30
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	158
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(89)80171-8
State	Published - Jan 16 1989

Funding

Supported by American Cancer Society Grant CD-190 and USPHS Grant CA-43906 and the Shriners Hospitals for Crippled Children. We thank Susan Bruno for secretarial support, Patrick Turk, and Dana Frederick for technical assistance and Drs. Thomas Stossel, Rex Chisholm and Ronald A. Malt for helpful discussions.

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry
Cell Biology

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10.1016/S0006-291X(89)80171-8

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title = "Alterations in c-abl gene methylation in cells transformed by phagocyte-generated oxidants",

abstract = "DNA from 10T1/2 cells transformed by activated neutrophils was analyzed for restriction length polymorphisms (RFLPs) in cellular homologues of retroviral oncogenes, and consistent RFLPs were found in MspI sites of the c-abl gene of all PMN-transformed cell lines. MspI digests probed with c-myc, v-Ki-ras, v-Ha-ras or v-mos showed no RFLPs, and none were observed in EcoRI, PstI, HindIII, BamHI, SmaI, Sau3a, MboI, HhaI, or TaqI digests probed with v-abl. Analysis of HpaII digests supports the conclusion that c-abl RFLPs result from differential methylation of the CCGG HpaII/MspI recognition sequence. MspI RFLPs in the c-abl gene may provide markers for oxidant-related genetic injury.",

author = "Weitzman, {Sigmund A.} and Lee, {Robert M.} and Ouellette, {Andre J.}",

note = "Funding Information: Supported by American Cancer Society Grant CD-190 and USPHS Grant CA-43906 and the Shriners Hospitals for Crippled Children. We thank Susan Bruno for secretarial support, Patrick Turk, and Dana Frederick for technical assistance and Drs. Thomas Stossel, Rex Chisholm and Ronald A. Malt for helpful discussions.",

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AU - Lee, Robert M.

AU - Ouellette, Andre J.

N1 - Funding Information: Supported by American Cancer Society Grant CD-190 and USPHS Grant CA-43906 and the Shriners Hospitals for Crippled Children. We thank Susan Bruno for secretarial support, Patrick Turk, and Dana Frederick for technical assistance and Drs. Thomas Stossel, Rex Chisholm and Ronald A. Malt for helpful discussions.

PY - 1989/1/16

Y1 - 1989/1/16

N2 - DNA from 10T1/2 cells transformed by activated neutrophils was analyzed for restriction length polymorphisms (RFLPs) in cellular homologues of retroviral oncogenes, and consistent RFLPs were found in MspI sites of the c-abl gene of all PMN-transformed cell lines. MspI digests probed with c-myc, v-Ki-ras, v-Ha-ras or v-mos showed no RFLPs, and none were observed in EcoRI, PstI, HindIII, BamHI, SmaI, Sau3a, MboI, HhaI, or TaqI digests probed with v-abl. Analysis of HpaII digests supports the conclusion that c-abl RFLPs result from differential methylation of the CCGG HpaII/MspI recognition sequence. MspI RFLPs in the c-abl gene may provide markers for oxidant-related genetic injury.

AB - DNA from 10T1/2 cells transformed by activated neutrophils was analyzed for restriction length polymorphisms (RFLPs) in cellular homologues of retroviral oncogenes, and consistent RFLPs were found in MspI sites of the c-abl gene of all PMN-transformed cell lines. MspI digests probed with c-myc, v-Ki-ras, v-Ha-ras or v-mos showed no RFLPs, and none were observed in EcoRI, PstI, HindIII, BamHI, SmaI, Sau3a, MboI, HhaI, or TaqI digests probed with v-abl. Analysis of HpaII digests supports the conclusion that c-abl RFLPs result from differential methylation of the CCGG HpaII/MspI recognition sequence. MspI RFLPs in the c-abl gene may provide markers for oxidant-related genetic injury.

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Alterations in c-abl gene methylation in cells transformed by phagocyte-generated oxidants

Abstract

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