Altered erythrocyte membrane phosphorylation in psoriasis

R. KUMAR*, VIRGINIA C. WEISS, D. P. WEST, LAURA A. CHIERO

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Autophosphorylation by [y‐32P]ATP of erythrocyte membranes from controls, psoriatic patients and patients with skin disorders other than psoriasis was compared by polyacrylamide gel electrophoresis. Compared with controls, membranes from psoriatic patients showed significantly less 32P incorporation in the band 2 region (nomenclature of Fairbanks et al., 1971). In addition, psoriasis and some of the other skin diseases examined displayed decreased phosphorylation in the region of bands 2.9‐3 and 4.5‐4.8. A new polypeptidc band in the 18‐20,000 dalton region was also observed in the diseases examined. Altered epidermal plasma membranes have been implicated in the pathogenesis of psoriasis, and our findings suggest the defective plasma membranes may be a generalized phenomenon in this disorder. Mahrle & Orfanos (1977) have suggested that psoriasis may be a result of alteration or failure in control mechanisms pertaining to the epidermal plasma membrane system. Evidence in support of this hypothesis comes from observations of functional deficiency in some of the plasma membrane‐associated enzymes and other components of skin. The most readily recognized alterations are in connection with the adenosine 3′,5′‐cyclic monophosphate (cyclic AMP) and guanosine 3′,5′‐cyclic monophosphate (cyclic GMP) balance (Voorhees & Duell, 1975). Although cyclic AMP levels are not altered in psoriatic skin (Adachi ei al., 1981,1980; Marcelo, et al., 1979), adenylate cyclase is modified in terms of its response to epinephrine, prostaglandin E, histamine, AMP, adenosine and sodium fluoride (Wright et al., 1973; lizuka et al., 1978), whereas the cyclic GMP level is increased (Adachi et al., 1981, 1980; Marcelo et al., 1979). Cytochemical methods have revealed a decrease in membrane‐bound ATPase and phosphomonoesterase activities in psoriatic epidermis (Mahrle & Orfanos, 1975) and notable changes in the distribution of compartmcntation of membrane‐bound glycoconjugates (Orfanos, Mahrle 6k Runne, 1976). Recently, an increased uptake of radioactively labelled sugars into the

Original languageEnglish (US)
Pages (from-to)267-275
Number of pages9
JournalBritish Journal of Dermatology
Volume109
Issue number3
DOIs
StatePublished - Jan 1 1983

ASJC Scopus subject areas

  • Dermatology

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