Altered expression of the DNA repair- protein, N-methylpurine-DNA glycosylase (MPG), in breast cancer

Sonia R. Cerda, Patrick W. Turk, Ann D. Thor, Sigmund A. Weitzman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

We examined expression of N-methylpurine-DNA glycosylase (MPG), a DNA repair enzyme that removes N-alkylpurine damage, in normal, malignant, and immortalized breast epithelial cells, and breast cancer cell lines (MDA-MB-231, MCF7, T47D). Northern analysis showed increased expression in cancer versus normal breast epithelial cells (2-24-fold). Southern blots revealed no gene amplification or polymorphisms. Immunofluorescence, immunohistochemistry, and Western blot analysis demonstrated increased MPG protein expression in the tumor cells that correlated with elevated glycosylase activity. Since MPG overexpression has been shown to be paradoxically associated with increased susceptibility to DNA damage, up-regulation of this gene may suggest a functional role in breast carcinogenesis.

Original languageEnglish (US)
Pages (from-to)12-18
Number of pages7
JournalFEBS Letters
Volume431
Issue number1
DOIs
StatePublished - Jul 10 1998

Keywords

  • Alkylpurine
  • Breast cancer
  • DNA repair
  • N-methylpurine-DNA glycosylase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Altered expression of the DNA repair- protein, N-methylpurine-DNA glycosylase (MPG), in breast cancer'. Together they form a unique fingerprint.

Cite this