Altered expression of the DNA repair- protein, N-methylpurine-DNA glycosylase (MPG), in breast cancer

Sonia R. Cerda; Patrick W. Turk; Ann D. Thor; Sigmund A. Weitzman

doi:10.1016/S0014-5793(98)00697-8

Altered expression of the DNA repair- protein, N-methylpurine-DNA glycosylase (MPG), in breast cancer

Sonia R. Cerda, Patrick W. Turk, Ann D. Thor, Sigmund A. Weitzman^*

^*Corresponding author for this work

Medicine, Hematology Oncology Division

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

We examined expression of N-methylpurine-DNA glycosylase (MPG), a DNA repair enzyme that removes N-alkylpurine damage, in normal, malignant, and immortalized breast epithelial cells, and breast cancer cell lines (MDA-MB-231, MCF7, T47D). Northern analysis showed increased expression in cancer versus normal breast epithelial cells (2-24-fold). Southern blots revealed no gene amplification or polymorphisms. Immunofluorescence, immunohistochemistry, and Western blot analysis demonstrated increased MPG protein expression in the tumor cells that correlated with elevated glycosylase activity. Since MPG overexpression has been shown to be paradoxically associated with increased susceptibility to DNA damage, up-regulation of this gene may suggest a functional role in breast carcinogenesis.

Original language	English (US)
Pages (from-to)	12-18
Number of pages	7
Journal	FEBS Letters
Volume	431
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(98)00697-8
State	Published - Jul 10 1998

Keywords

Alkylpurine
Breast cancer
DNA repair
N-methylpurine-DNA glycosylase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/S0014-5793(98)00697-8

Fingerprint Dive into the research topics of 'Altered expression of the DNA repair- protein, N-methylpurine-DNA glycosylase (MPG), in breast cancer'. Together they form a unique fingerprint.

Cite this

@article{060c4f17d32747c1bebde4ddb2e0b1ae,

title = "Altered expression of the DNA repair- protein, N-methylpurine-DNA glycosylase (MPG), in breast cancer",

abstract = "We examined expression of N-methylpurine-DNA glycosylase (MPG), a DNA repair enzyme that removes N-alkylpurine damage, in normal, malignant, and immortalized breast epithelial cells, and breast cancer cell lines (MDA-MB-231, MCF7, T47D). Northern analysis showed increased expression in cancer versus normal breast epithelial cells (2-24-fold). Southern blots revealed no gene amplification or polymorphisms. Immunofluorescence, immunohistochemistry, and Western blot analysis demonstrated increased MPG protein expression in the tumor cells that correlated with elevated glycosylase activity. Since MPG overexpression has been shown to be paradoxically associated with increased susceptibility to DNA damage, up-regulation of this gene may suggest a functional role in breast carcinogenesis.",

keywords = "Alkylpurine, Breast cancer, DNA repair, N-methylpurine-DNA glycosylase",

author = "Cerda, {Sonia R.} and Turk, {Patrick W.} and Thor, {Ann D.} and Weitzman, {Sigmund A.}",

year = "1998",

month = jul,

day = "10",

doi = "10.1016/S0014-5793(98)00697-8",

language = "English (US)",

volume = "431",

pages = "12--18",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Altered expression of the DNA repair- protein, N-methylpurine-DNA glycosylase (MPG), in breast cancer

AU - Cerda, Sonia R.

AU - Turk, Patrick W.

AU - Thor, Ann D.

AU - Weitzman, Sigmund A.

PY - 1998/7/10

Y1 - 1998/7/10

N2 - We examined expression of N-methylpurine-DNA glycosylase (MPG), a DNA repair enzyme that removes N-alkylpurine damage, in normal, malignant, and immortalized breast epithelial cells, and breast cancer cell lines (MDA-MB-231, MCF7, T47D). Northern analysis showed increased expression in cancer versus normal breast epithelial cells (2-24-fold). Southern blots revealed no gene amplification or polymorphisms. Immunofluorescence, immunohistochemistry, and Western blot analysis demonstrated increased MPG protein expression in the tumor cells that correlated with elevated glycosylase activity. Since MPG overexpression has been shown to be paradoxically associated with increased susceptibility to DNA damage, up-regulation of this gene may suggest a functional role in breast carcinogenesis.

AB - We examined expression of N-methylpurine-DNA glycosylase (MPG), a DNA repair enzyme that removes N-alkylpurine damage, in normal, malignant, and immortalized breast epithelial cells, and breast cancer cell lines (MDA-MB-231, MCF7, T47D). Northern analysis showed increased expression in cancer versus normal breast epithelial cells (2-24-fold). Southern blots revealed no gene amplification or polymorphisms. Immunofluorescence, immunohistochemistry, and Western blot analysis demonstrated increased MPG protein expression in the tumor cells that correlated with elevated glycosylase activity. Since MPG overexpression has been shown to be paradoxically associated with increased susceptibility to DNA damage, up-regulation of this gene may suggest a functional role in breast carcinogenesis.

KW - Alkylpurine

KW - Breast cancer

KW - DNA repair

KW - N-methylpurine-DNA glycosylase

UR - http://www.scopus.com/inward/record.url?scp=0032504048&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032504048&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(98)00697-8

DO - 10.1016/S0014-5793(98)00697-8

M3 - Article

C2 - 9684856

AN - SCOPUS:0032504048

VL - 431

SP - 12

EP - 18

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1

ER -