Alternatively spliced exons encode the tissue-specific 5′ termini of leukocyte pp52 and stromal cell S37 mRNA isoforms

Alexis A. Thompson, Sidne A. Omori, Michael J. Gilly, William May, Melinda S. Gordon, William J. Wood, Erina Miyoshi, Cindy Sue Malone, Jeff Gimble, Christopher T. Denny, Randolph Wall*

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The pp52 gene encodes an intracellular, F-actin-binding phosphoprotein (also designated LSP1 and WP34) postulated to function in cytoskeleton dynamics and cell motility. We previously reported that different mRNA isoforms are expressed from this gene in cells of the leukocyte lineage versus mesodermally derived cells. These tissue-specific mRNA isoforms are identical except for 5′-untranslated regions and sequences coding for unique N-termini of 23 and 21 amino acids, respectively. As this is a single-copy gene, we predicted that these tissue-specific mRNA isoforms would be generated by alternative RNA splicing. We report that the unique 5′ sequences in these mRNA isoforms are encoded in two separate exons containing ATG initiation codons. These features confirm that the pp52 and S37 mRNA isoforms are generated by alternative RNA splicing and establish that they are independently translated. Other results presented here indicate that the differential expression of these exons in leukocytes versus mesodermally derived cells is regulated at the level of transcription by tissue-specific promoters.

Original languageEnglish (US)
Pages (from-to)352-357
Number of pages6
JournalGenomics
Volume32
Issue number3
DOIs
StatePublished - Mar 15 1996

ASJC Scopus subject areas

  • Genetics

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    Thompson, A. A., Omori, S. A., Gilly, M. J., May, W., Gordon, M. S., Wood, W. J., Miyoshi, E., Malone, C. S., Gimble, J., Denny, C. T., & Wall, R. (1996). Alternatively spliced exons encode the tissue-specific 5′ termini of leukocyte pp52 and stromal cell S37 mRNA isoforms. Genomics, 32(3), 352-357. https://doi.org/10.1006/geno.1996.0129