A search of protein databases revealed amino acid homologies among human biotinidase, bacterial aliphatic amidases, and bacterial and plant nitrilases. Amino acids YRK210-212 of biotinidase are conserved among the enzyme families. This homology and naturally occurring mutations that cause biotinidase deficiency suggest that this region is essential for enzyme activity land is conserved from bacteria. Cys245 is likely the cysteine in the active site of biotinidase. (C) 2000 Academic Press.
- Active site
- Aliphatic amidase
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Molecular Biology