AMPK interacts with DSCAM and plays an important role in Netrin-1 induced neurite outgrowth

Kun Zhu, Xiaoping Chen, Jianghong Liu, Haihong Ye, Li Zhu*, Jane Y. Wu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Down syndrome cell adhesion molecule (DSCAM) acts as a netrin-1 receptor and mediates attractive response of axons to netrin-1 in neural development. However, the signaling mechanisms of netrin-DSCAM remain unclear. Here we report that AMP-activated protein kinase (AMPK) interacts with DSCAM through its γ subunit, but does not interact with DCC (deleted in colorectal cancer), another major receptor for netrin-1. Netrin-treatment of cultured cortical neurons leads to increased phosphorylation of AMPK. Both AMPK mutant with dominant-negative effect and AMPK inhibitor can significantly suppress netrin-1 induced neurite outgrowth. Together, these findings demonstrate that AMPK interacts with DSCAM and plays an important role in netrin-1 induced neurite outgrowth. Our study uncovers a previously unknown component, AMPK, in netrin-DSCAM signaling pathway.

Original languageEnglish (US)
Pages (from-to)155-161
Number of pages7
JournalProtein and Cell
Volume4
Issue number2
DOIs
StatePublished - Feb 2013

Keywords

  • AMP-activated protein kinase (AMPK)
  • Down syndrome cell adhesion molecule (DSCAM)
  • netrin
  • neurite outgrowth

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Drug Discovery
  • Cell Biology

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