Amplification of MGC2177, PLAG1, PSMC6P, and LYN in a malignant mixed tumor of salivary gland detected by cDNA microarray with tyramide signal amplification

Yvonne T M Tsang, Yi Mieng Chang, Xinyan Lu, Pulivarthi H. Rao, Ching C. Lau, Kwong Kwok Wong*

*Corresponding author for this work

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Gene amplifications have been observed in many different tumor cells, and many of these changes are related to tumor pathogenesis. Comparative genomic hybridization (CGH) using metaphase chromosomes can detect changes in chromosome copy number with a resolution of 10-20 Mb. Current advances in CGH analysis in a microarray format allow us to refine such changes down to the gene level. We applied microarray technology to detect novel gene amplification in a malignant mixed tumor of salivary gland. Besides detecting previously known gene amplifications (MDM2 and MYC), we identified four other highly amplified genes located at 8q11.2∼q13: MGC2177, PLAG1, PSMC6P, and LYN. The amplification was further validated with real-time quantitative polymerase chain reaction.

Original languageEnglish (US)
Pages (from-to)124-128
Number of pages5
JournalCancer Genetics and Cytogenetics
Volume152
Issue number2
DOIs
StatePublished - Jul 15 2004

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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