Amplification of MGC2177, PLAG1, PSMC6P, and LYN in a malignant mixed tumor of salivary gland detected by cDNA microarray with tyramide signal amplification

Yvonne T M Tsang; Yi Mieng Chang; Xinyan Lu; Pulivarthi H. Rao; Ching C. Lau; Kwong Kwok Wong

doi:10.1016/j.cancergencyto.2003.12.001

Amplification of MGC2177, PLAG1, PSMC6P, and LYN in a malignant mixed tumor of salivary gland detected by cDNA microarray with tyramide signal amplification

Yvonne T M Tsang, Yi Mieng Chang, Xinyan Lu, Pulivarthi H. Rao, Ching C. Lau, Kwong Kwok Wong^*

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Gene amplifications have been observed in many different tumor cells, and many of these changes are related to tumor pathogenesis. Comparative genomic hybridization (CGH) using metaphase chromosomes can detect changes in chromosome copy number with a resolution of 10-20 Mb. Current advances in CGH analysis in a microarray format allow us to refine such changes down to the gene level. We applied microarray technology to detect novel gene amplification in a malignant mixed tumor of salivary gland. Besides detecting previously known gene amplifications (MDM2 and MYC), we identified four other highly amplified genes located at 8q11.2∼q13: MGC2177, PLAG1, PSMC6P, and LYN. The amplification was further validated with real-time quantitative polymerase chain reaction.

Original language	English (US)
Pages (from-to)	124-128
Number of pages	5
Journal	Cancer Genetics and Cytogenetics
Volume	152
Issue number	2
DOIs	https://doi.org/10.1016/j.cancergencyto.2003.12.001
State	Published - Jul 15 2004

ASJC Scopus subject areas

Molecular Biology
Genetics
Cancer Research

UN SDGs

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10.1016/j.cancergencyto.2003.12.001

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@article{044447d3534a414fb6878a21f5556ae7,

title = "Amplification of MGC2177, PLAG1, PSMC6P, and LYN in a malignant mixed tumor of salivary gland detected by cDNA microarray with tyramide signal amplification",

abstract = "Gene amplifications have been observed in many different tumor cells, and many of these changes are related to tumor pathogenesis. Comparative genomic hybridization (CGH) using metaphase chromosomes can detect changes in chromosome copy number with a resolution of 10-20 Mb. Current advances in CGH analysis in a microarray format allow us to refine such changes down to the gene level. We applied microarray technology to detect novel gene amplification in a malignant mixed tumor of salivary gland. Besides detecting previously known gene amplifications (MDM2 and MYC), we identified four other highly amplified genes located at 8q11.2∼q13: MGC2177, PLAG1, PSMC6P, and LYN. The amplification was further validated with real-time quantitative polymerase chain reaction.",

author = "Tsang, {Yvonne T M} and Chang, {Yi Mieng} and Xinyan Lu and Rao, {Pulivarthi H.} and Lau, {Ching C.} and Wong, {Kwong Kwok}",

note = "Funding Information: The work is partly supported by grants from John Dunn Foundation and Kleberg Foundation to the Cancer Genomics program. We are also indebted to Spectral Genomics, Inc. (Houston, TX), for the BAC array and to Rita Cheng for critical reading of the manuscript.",

year = "2004",

month = jul,

day = "15",

doi = "10.1016/j.cancergencyto.2003.12.001",

language = "English (US)",

volume = "152",

pages = "124--128",

journal = "Cancer Genetics and Cytogenetics",

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publisher = "Elsevier Inc.",

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TY - JOUR

T1 - Amplification of MGC2177, PLAG1, PSMC6P, and LYN in a malignant mixed tumor of salivary gland detected by cDNA microarray with tyramide signal amplification

AU - Tsang, Yvonne T M

AU - Chang, Yi Mieng

AU - Lu, Xinyan

AU - Rao, Pulivarthi H.

AU - Lau, Ching C.

AU - Wong, Kwong Kwok

N1 - Funding Information: The work is partly supported by grants from John Dunn Foundation and Kleberg Foundation to the Cancer Genomics program. We are also indebted to Spectral Genomics, Inc. (Houston, TX), for the BAC array and to Rita Cheng for critical reading of the manuscript.

PY - 2004/7/15

Y1 - 2004/7/15

N2 - Gene amplifications have been observed in many different tumor cells, and many of these changes are related to tumor pathogenesis. Comparative genomic hybridization (CGH) using metaphase chromosomes can detect changes in chromosome copy number with a resolution of 10-20 Mb. Current advances in CGH analysis in a microarray format allow us to refine such changes down to the gene level. We applied microarray technology to detect novel gene amplification in a malignant mixed tumor of salivary gland. Besides detecting previously known gene amplifications (MDM2 and MYC), we identified four other highly amplified genes located at 8q11.2∼q13: MGC2177, PLAG1, PSMC6P, and LYN. The amplification was further validated with real-time quantitative polymerase chain reaction.

AB - Gene amplifications have been observed in many different tumor cells, and many of these changes are related to tumor pathogenesis. Comparative genomic hybridization (CGH) using metaphase chromosomes can detect changes in chromosome copy number with a resolution of 10-20 Mb. Current advances in CGH analysis in a microarray format allow us to refine such changes down to the gene level. We applied microarray technology to detect novel gene amplification in a malignant mixed tumor of salivary gland. Besides detecting previously known gene amplifications (MDM2 and MYC), we identified four other highly amplified genes located at 8q11.2∼q13: MGC2177, PLAG1, PSMC6P, and LYN. The amplification was further validated with real-time quantitative polymerase chain reaction.

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DO - 10.1016/j.cancergencyto.2003.12.001

M3 - Article

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AN - SCOPUS:3242704957

SN - 0165-4608

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SP - 124

EP - 128

JO - Cancer Genetics and Cytogenetics

JF - Cancer Genetics and Cytogenetics

IS - 2

ER -

Amplification of MGC2177, PLAG1, PSMC6P, and LYN in a malignant mixed tumor of salivary gland detected by cDNA microarray with tyramide signal amplification

Abstract

ASJC Scopus subject areas

UN SDGs

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