Amplificação do gene flge fornece evidência para a existência de uma borreliose Brasileira

Translated title of the contribution: Amplification of the flgE gene provides evidence for the existence of a Brazilian borreliosis

Elenice Mantovani, Roberta G. Marangoni, Giancarla Gauditano, Virgínia L N Bonoldi, Natalino H. Yoshinari

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Introduction: The symptoms of Brazilian borreliosis resemble the clinical manifestations of Lyme disease (LD). However, there are differences between the two in terms of epidemiological and laboratory findings. Primers usually employed to diagnose LD have failed to detect Borrelia strains in Brazil. Objective: We aimed to identify the Brazilian Borrelia using a conserved gene that synthesizes the flagellar hook (flgE) of Borrelia burgdorferi sensu lato. Method: Three patients presenting with erythema migrans and positive epidemiological histories were recruited for the study. Blood samples were collected, and the DNA was extracted by commercial kits. Results: The gene flgE was amplified from DNA of all selected patients. Upon sequencing, these positive samples revealed 99% homology to B. burgdorferi flgE. Conclusion: These results support the existence of borreliosis in Brazil. However, it is unclear whether this borreliosis is caused by a genetically modified B. burgdorferi sensu stricto or by a new species of Borrelia spp.

Translated title of the contributionAmplification of the flgE gene provides evidence for the existence of a Brazilian borreliosis
Original languagePortuguese
Pages (from-to)153-157
Number of pages5
JournalRevista do Instituto de Medicina Tropical de Sao Paulo
Volume54
Issue number3
DOIs
StatePublished - May 2012

Keywords

  • Lyme disease
  • Lyme disease-like
  • Spirochaetales

ASJC Scopus subject areas

  • Infectious Diseases

Fingerprint Dive into the research topics of 'Amplification of the flgE gene provides evidence for the existence of a Brazilian borreliosis'. Together they form a unique fingerprint.

Cite this