An acetylatable lysine controls CRP function in E. coli

Robert Davis, Ana Écija-Conesa, Julia Gallego-Jara, Teresa de Diego, Ekaterina V. Filippova, Gina Kuffel, Wayne F Anderson, Bradford W. Gibson, Birgit Schilling, Manuel Canovas*, Alan J. Wolfe

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Transcriptional regulation is the key to ensuring that proteins are expressed at the proper time and the proper amount. In Escherichia coli, the transcription factor cAMP receptor protein (CRP) is responsible for much of this regulation. Questions remain, however, regarding the regulation of CRP activity itself. Here, we demonstrate that a lysine (K100) on the surface of CRP has a dual function: to promote CRP activity at Class II promoters, and to ensure proper CRP steady state levels. Both functions require the lysine's positive charge; intriguingly, the positive charge of K100 can be neutralized by acetylation using the central metabolite acetyl phosphate as the acetyl donor. We propose that CRP K100 acetylation could be a mechanism by which the cell downwardly tunes CRP-dependent Class II promoter activity, whilst elevating CRP steady state levels, thus indirectly increasing Class I promoter activity. This mechanism would operate under conditions that favor acetate fermentation, such as during growth on glucose as the sole carbon source or when carbon flux exceeds the capacity of the central metabolic pathways.

Original languageEnglish (US)
Pages (from-to)116-131
Number of pages16
JournalMolecular Microbiology
Volume107
Issue number1
DOIs
StatePublished - Jan 1 2018

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Cyclic AMP Receptor Protein
Lysine
Escherichia coli
Acetylation
Carbon Cycle
Metabolic Networks and Pathways
Fermentation
Acetates
Transcription Factors
Carbon
Glucose

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Davis, R., Écija-Conesa, A., Gallego-Jara, J., de Diego, T., Filippova, E. V., Kuffel, G., ... Wolfe, A. J. (2018). An acetylatable lysine controls CRP function in E. coli. Molecular Microbiology, 107(1), 116-131. https://doi.org/10.1111/mmi.13874
Davis, Robert ; Écija-Conesa, Ana ; Gallego-Jara, Julia ; de Diego, Teresa ; Filippova, Ekaterina V. ; Kuffel, Gina ; Anderson, Wayne F ; Gibson, Bradford W. ; Schilling, Birgit ; Canovas, Manuel ; Wolfe, Alan J. / An acetylatable lysine controls CRP function in E. coli. In: Molecular Microbiology. 2018 ; Vol. 107, No. 1. pp. 116-131.
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Davis, R, Écija-Conesa, A, Gallego-Jara, J, de Diego, T, Filippova, EV, Kuffel, G, Anderson, WF, Gibson, BW, Schilling, B, Canovas, M & Wolfe, AJ 2018, 'An acetylatable lysine controls CRP function in E. coli', Molecular Microbiology, vol. 107, no. 1, pp. 116-131. https://doi.org/10.1111/mmi.13874

An acetylatable lysine controls CRP function in E. coli. / Davis, Robert; Écija-Conesa, Ana; Gallego-Jara, Julia; de Diego, Teresa; Filippova, Ekaterina V.; Kuffel, Gina; Anderson, Wayne F; Gibson, Bradford W.; Schilling, Birgit; Canovas, Manuel; Wolfe, Alan J.

In: Molecular Microbiology, Vol. 107, No. 1, 01.01.2018, p. 116-131.

Research output: Contribution to journalArticle

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AU - Anderson, Wayne F

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Davis R, Écija-Conesa A, Gallego-Jara J, de Diego T, Filippova EV, Kuffel G et al. An acetylatable lysine controls CRP function in E. coli. Molecular Microbiology. 2018 Jan 1;107(1):116-131. https://doi.org/10.1111/mmi.13874