TY - JOUR
T1 - An amino-terminal fragment of urokinase isolated from a prostate cancer cell line (PC-3) is mitogenic for osteoblast-like cells
AU - Rabbani, Shafast A.
AU - Desjardins, Johanne
AU - Bell, Alexander W.
AU - Banville, Denis
AU - Mazar, Andrew
AU - Henkin, Jack
AU - Goltzman, David
N1 - Funding Information:
We thank I. Bolivar, F. Dumas and B. Gibbs for technical assistance and D. Allen for secreterial assistance. This work was supported by National Institute of Health Grant ROI CA 37126 and by Medical Research Council of Canada Grants MT-5775 and MT-10630.
PY - 1990/12/31
Y1 - 1990/12/31
N2 - A peptide mitogen for cultured osteoblast-like cells was purified from aerum-free conditioned culture medium of a human prostatic cancer cell line, PC-3. Based on amino acid sequencing and estimated molecular weight, this peptide was identified as an NH2-terminal fragment of urokinase-type plasminogen activator (uPA). Recombinant high molecular weight (HMW) uPA and the NH2-terminal growth factor domain (GFD) of uPA, but not low molecular weight (LMW) uPA (lacking the NH2-terminal region) stimulated [3H]thymidine incorporation and proliferation in osteoblast-like cells, and specific, competitive binding sites for HMW, but not LMW, uPA were demonstrable. These studies demonstrate the production of a mitogenic NH2-terminal fragment of uPA by a human prostatic cancer cell line which may be of importance in the pathogenesis of osteoblastic metastases.
AB - A peptide mitogen for cultured osteoblast-like cells was purified from aerum-free conditioned culture medium of a human prostatic cancer cell line, PC-3. Based on amino acid sequencing and estimated molecular weight, this peptide was identified as an NH2-terminal fragment of urokinase-type plasminogen activator (uPA). Recombinant high molecular weight (HMW) uPA and the NH2-terminal growth factor domain (GFD) of uPA, but not low molecular weight (LMW) uPA (lacking the NH2-terminal region) stimulated [3H]thymidine incorporation and proliferation in osteoblast-like cells, and specific, competitive binding sites for HMW, but not LMW, uPA were demonstrable. These studies demonstrate the production of a mitogenic NH2-terminal fragment of uPA by a human prostatic cancer cell line which may be of importance in the pathogenesis of osteoblastic metastases.
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U2 - 10.1016/S0006-291X(05)80893-9
DO - 10.1016/S0006-291X(05)80893-9
M3 - Article
C2 - 2125213
AN - SCOPUS:0025543131
VL - 173
SP - 1058
EP - 1064
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -