An amino-terminal fragment of urokinase isolated from a prostate cancer cell line (PC-3) is mitogenic for osteoblast-like cells

Shafast A. Rabbani; Johanne Desjardins; Alexander W. Bell; Denis Banville; Andrew Mazar; Jack Henkin; David Goltzman

doi:10.1016/S0006-291X(05)80893-9

An amino-terminal fragment of urokinase isolated from a prostate cancer cell line (PC-3) is mitogenic for osteoblast-like cells

Shafast A. Rabbani, Johanne Desjardins, Alexander W. Bell, Denis Banville, Andrew Mazar, Jack Henkin, David Goltzman^*

^*Corresponding author for this work

CLP - Chemistry of Life Processes Institute

Research output: Contribution to journal › Article › peer-review

185 Scopus citations

Abstract

A peptide mitogen for cultured osteoblast-like cells was purified from aerum-free conditioned culture medium of a human prostatic cancer cell line, PC-3. Based on amino acid sequencing and estimated molecular weight, this peptide was identified as an NH₂-terminal fragment of urokinase-type plasminogen activator (uPA). Recombinant high molecular weight (HMW) uPA and the NH₂-terminal growth factor domain (GFD) of uPA, but not low molecular weight (LMW) uPA (lacking the NH₂-terminal region) stimulated [³H]thymidine incorporation and proliferation in osteoblast-like cells, and specific, competitive binding sites for HMW, but not LMW, uPA were demonstrable. These studies demonstrate the production of a mitogenic NH₂-terminal fragment of uPA by a human prostatic cancer cell line which may be of importance in the pathogenesis of osteoblastic metastases.

Original language	English (US)
Pages (from-to)	1058-1064
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	173
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(05)80893-9
State	Published - Dec 31 1990

Funding

We thank I. Bolivar, F. Dumas and B. Gibbs for technical assistance and D. Allen for secreterial assistance. This work was supported by National Institute of Health Grant ROI CA 37126 and by Medical Research Council of Canada Grants MT-5775 and MT-10630.

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(05)80893-9

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title = "An amino-terminal fragment of urokinase isolated from a prostate cancer cell line (PC-3) is mitogenic for osteoblast-like cells",

abstract = "A peptide mitogen for cultured osteoblast-like cells was purified from aerum-free conditioned culture medium of a human prostatic cancer cell line, PC-3. Based on amino acid sequencing and estimated molecular weight, this peptide was identified as an NH2-terminal fragment of urokinase-type plasminogen activator (uPA). Recombinant high molecular weight (HMW) uPA and the NH2-terminal growth factor domain (GFD) of uPA, but not low molecular weight (LMW) uPA (lacking the NH2-terminal region) stimulated [3H]thymidine incorporation and proliferation in osteoblast-like cells, and specific, competitive binding sites for HMW, but not LMW, uPA were demonstrable. These studies demonstrate the production of a mitogenic NH2-terminal fragment of uPA by a human prostatic cancer cell line which may be of importance in the pathogenesis of osteoblastic metastases.",

author = "Rabbani, {Shafast A.} and Johanne Desjardins and Bell, {Alexander W.} and Denis Banville and Andrew Mazar and Jack Henkin and David Goltzman",

note = "Funding Information: We thank I. Bolivar, F. Dumas and B. Gibbs for technical assistance and D. Allen for secreterial assistance. This work was supported by National Institute of Health Grant ROI CA 37126 and by Medical Research Council of Canada Grants MT-5775 and MT-10630.",

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AU - Rabbani, Shafast A.

AU - Desjardins, Johanne

AU - Bell, Alexander W.

AU - Banville, Denis

AU - Mazar, Andrew

AU - Henkin, Jack

AU - Goltzman, David

N1 - Funding Information: We thank I. Bolivar, F. Dumas and B. Gibbs for technical assistance and D. Allen for secreterial assistance. This work was supported by National Institute of Health Grant ROI CA 37126 and by Medical Research Council of Canada Grants MT-5775 and MT-10630.

PY - 1990/12/31

Y1 - 1990/12/31

N2 - A peptide mitogen for cultured osteoblast-like cells was purified from aerum-free conditioned culture medium of a human prostatic cancer cell line, PC-3. Based on amino acid sequencing and estimated molecular weight, this peptide was identified as an NH2-terminal fragment of urokinase-type plasminogen activator (uPA). Recombinant high molecular weight (HMW) uPA and the NH2-terminal growth factor domain (GFD) of uPA, but not low molecular weight (LMW) uPA (lacking the NH2-terminal region) stimulated [3H]thymidine incorporation and proliferation in osteoblast-like cells, and specific, competitive binding sites for HMW, but not LMW, uPA were demonstrable. These studies demonstrate the production of a mitogenic NH2-terminal fragment of uPA by a human prostatic cancer cell line which may be of importance in the pathogenesis of osteoblastic metastases.

AB - A peptide mitogen for cultured osteoblast-like cells was purified from aerum-free conditioned culture medium of a human prostatic cancer cell line, PC-3. Based on amino acid sequencing and estimated molecular weight, this peptide was identified as an NH2-terminal fragment of urokinase-type plasminogen activator (uPA). Recombinant high molecular weight (HMW) uPA and the NH2-terminal growth factor domain (GFD) of uPA, but not low molecular weight (LMW) uPA (lacking the NH2-terminal region) stimulated [3H]thymidine incorporation and proliferation in osteoblast-like cells, and specific, competitive binding sites for HMW, but not LMW, uPA were demonstrable. These studies demonstrate the production of a mitogenic NH2-terminal fragment of uPA by a human prostatic cancer cell line which may be of importance in the pathogenesis of osteoblastic metastases.

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An amino-terminal fragment of urokinase isolated from a prostate cancer cell line (PC-3) is mitogenic for osteoblast-like cells

Abstract

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UN SDGs

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