An analysis of the role of serum in parathyroid hormone-induced bone resorption in tissue culture

Paula H. Stern; L. G. Raisz

doi:10.1016/0014-4827(67)90413-2

An analysis of the role of serum in parathyroid hormone-induced bone resorption in tissue culture

Paula H. Stern^*, L. G. Raisz

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

1. 1. Several chemically-defined media were tested for their capacity to support parathyroid hormone-induced ⁴⁵Ca release from embryonic bone. None was completely satisfactory. 2. 2. A medium consisting of 2 per cent serum in CMRL 1066 would support the parathyroid hormone effect as well as did media containing 50 per cent serum plus 50 per cent CMRL 1066 or 50 per cent Eagle Basal Medium. 3. 3. The albumin content of rat serum could be substituted for the whole serum without loss of the observed parathyroid hormone effect. Different albumin preparations varied in their capacity to support the response. Serum globulins, gelatin, histone, heparin or insulin could not replace serum. 4. 4. A number of substances enhanced ⁴⁵Ca release in the absence of parathyroid hormone. Bovine albumin (fraction V) was the most effective preparation tested.

Original language	English (US)
Pages (from-to)	106-120
Number of pages	15
Journal	Experimental Cell Research
Volume	46
Issue number	1
DOIs	https://doi.org/10.1016/0014-4827(67)90413-2
State	Published - Apr 1967

ASJC Scopus subject areas

Cell Biology

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10.1016/0014-4827(67)90413-2

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abstract = "1. 1. Several chemically-defined media were tested for their capacity to support parathyroid hormone-induced 45Ca release from embryonic bone. None was completely satisfactory. 2. 2. A medium consisting of 2 per cent serum in CMRL 1066 would support the parathyroid hormone effect as well as did media containing 50 per cent serum plus 50 per cent CMRL 1066 or 50 per cent Eagle Basal Medium. 3. 3. The albumin content of rat serum could be substituted for the whole serum without loss of the observed parathyroid hormone effect. Different albumin preparations varied in their capacity to support the response. Serum globulins, gelatin, histone, heparin or insulin could not replace serum. 4. 4. A number of substances enhanced 45Ca release in the absence of parathyroid hormone. Bovine albumin (fraction V) was the most effective preparation tested.",

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N2 - 1. 1. Several chemically-defined media were tested for their capacity to support parathyroid hormone-induced 45Ca release from embryonic bone. None was completely satisfactory. 2. 2. A medium consisting of 2 per cent serum in CMRL 1066 would support the parathyroid hormone effect as well as did media containing 50 per cent serum plus 50 per cent CMRL 1066 or 50 per cent Eagle Basal Medium. 3. 3. The albumin content of rat serum could be substituted for the whole serum without loss of the observed parathyroid hormone effect. Different albumin preparations varied in their capacity to support the response. Serum globulins, gelatin, histone, heparin or insulin could not replace serum. 4. 4. A number of substances enhanced 45Ca release in the absence of parathyroid hormone. Bovine albumin (fraction V) was the most effective preparation tested.

AB - 1. 1. Several chemically-defined media were tested for their capacity to support parathyroid hormone-induced 45Ca release from embryonic bone. None was completely satisfactory. 2. 2. A medium consisting of 2 per cent serum in CMRL 1066 would support the parathyroid hormone effect as well as did media containing 50 per cent serum plus 50 per cent CMRL 1066 or 50 per cent Eagle Basal Medium. 3. 3. The albumin content of rat serum could be substituted for the whole serum without loss of the observed parathyroid hormone effect. Different albumin preparations varied in their capacity to support the response. Serum globulins, gelatin, histone, heparin or insulin could not replace serum. 4. 4. A number of substances enhanced 45Ca release in the absence of parathyroid hormone. Bovine albumin (fraction V) was the most effective preparation tested.

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An analysis of the role of serum in parathyroid hormone-induced bone resorption in tissue culture

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