An atlas of transcribed human cardiac promoters and enhancers reveals an important role of regulatory elements in heart failure

Ruslan M. Deviatiiarov, Anna Gams, Ivan V. Kulakovskiy, Andrey Buyan, Georgy Meshcheryakov, Roman Syunyaev, Ramesh Singh, Palak Shah, Tatiana V. Tatarinova*, Oleg Gusev*, Igor R. Efimov*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

A deeper knowledge of the dynamic transcriptional activity of promoters and enhancers is needed to improve mechanistic understanding of the pathogenesis of heart failure and heart diseases. In this study, we used cap analysis of gene expression (CAGE) to identify and quantify the activity of transcribed regulatory elements (TREs) in the four cardiac chambers of 21 healthy and ten failing adult human hearts. We identified 17,668 promoters and 14,920 enhancers associated with the expression of 14,519 genes. We showed how these regulatory elements are alternatively transcribed in different heart regions, in healthy versus failing hearts and in ischemic versus non-ischemic heart failure samples. Cardiac-disease-related single-nucleotide polymorphisms (SNPs) appeared to be enriched in TREs, potentially affecting the allele-specific transcription factor binding. To conclude, our open-source heart CAGE atlas will serve the cardiovascular community in improving the understanding of the role of the cardiac gene regulatory networks in cardiovascular disease and therapy.

Original languageEnglish (US)
Pages (from-to)58-75
Number of pages18
JournalNature Cardiovascular Research
Volume2
Issue number1
DOIs
StatePublished - Jan 2023

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Cell Biology
  • Medicine (miscellaneous)
  • Cardiology and Cardiovascular Medicine

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