An automated procedure for measuring biotinidase activity in serum

K. A. Weissbecker, H. D. Gruemer, G. S. Heard, W. G. Miller, W. E. Nance, B. Wolf

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


In this automated procedure for quantifying biotinidase activity in human serum, a manual colorimetric method that measures conversion of the enzyme's artificial substrate N-biotinyl p-aminobenzoate was modified for use with a Technicon AutoAnalyzer ll. The intra-run replicate precision (CV) was 2.1% and the day-to-day CV was 4.6% for quality-control sera. Results were linearly related to biotinidase activity in serum over the complete range of clinically relevant values, 0.2 to 11.0 U/L. Moreover, results of the automated assay were not significantly different from those of the manual assay. Because the automated procedure is faster and more precise, we recommend it for population-based studies and some screening studies.

Original languageEnglish (US)
Pages (from-to)831-833
Number of pages3
JournalClinical chemistry
Issue number5
StatePublished - 1989

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical


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