An electrochemical surface-enhanced Raman spectroscopy approach to anthrax detection

Xiaoyu Zhang; Chanda Ranjit Yonzon; Richard P. Van Duyne

doi:10.1117/12.508519

An electrochemical surface-enhanced Raman spectroscopy approach to anthrax detection

Xiaoyu Zhang^*, Chanda Ranjit Yonzon, Richard P. Van Duyne

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Conference article › peer-review

22 Scopus citations

Abstract

Metal film over nanosphere (MFON) electrodes are excellent substrates for surface-enhanced Raman scattering (SERS) spectroscopy. These surfaces are produced by vapor deposition of a metal film over nanospheres that are assembled in a hexagonally close packed arrangement. The efficiency and reproducibility of AgFON electrode as SERS substrates are confirmed by the repeatability of the electrochemical surface enhanced Raman scattering spectra of pyridine and the Ru(bpy)3 ³⁺/Ru(bpy)3 ²⁺ complexes adsorbed on AgFON electrodes. The Raman signal for AgFON electrodes is observed to be extremely stable even at extremely negative potentials in both aqueous and nonaqueous electrolytes. Recent reports have indicated that SERS enhancement factors of up to 14 orders of magnitude can be achieved, providing the sensitivity requisite for ultra trace level detection of target analytes. For this reason, we are developing a method for bacterial endospore SERS detection based on the endospores marker - dipicolinic acid (DPA). The SERS spectra of dipicolinic acid in aqueous solutions are reported. The dipicolinate vibrational features could be observed in the SERS spectra at the concentration as low as 8 × 10 ^-5 M in 5 minutes. These limits of detection are entirely controlled by the thermodynamics and kinetics of DPA binding to the AgFON surface.

Original language	English (US)
Pages (from-to)	82-91
Number of pages	10
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	5221
DOIs	https://doi.org/10.1117/12.508519
State	Published - Dec 1 2003
Event	Plasmonics: Metallic Nanostuctures and Their Optical Properties - San Diego, CA, United States Duration: Aug 3 2003 → Aug 5 2003

Keywords

Ag film over nanosphere
Anthrax
Dipicolinic acid
Electrochemistry
Endospore
Surface-enhanced Raman

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.508519

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title = "An electrochemical surface-enhanced Raman spectroscopy approach to anthrax detection",

abstract = "Metal film over nanosphere (MFON) electrodes are excellent substrates for surface-enhanced Raman scattering (SERS) spectroscopy. These surfaces are produced by vapor deposition of a metal film over nanospheres that are assembled in a hexagonally close packed arrangement. The efficiency and reproducibility of AgFON electrode as SERS substrates are confirmed by the repeatability of the electrochemical surface enhanced Raman scattering spectra of pyridine and the Ru(bpy)3 3+/Ru(bpy)3 2+ complexes adsorbed on AgFON electrodes. The Raman signal for AgFON electrodes is observed to be extremely stable even at extremely negative potentials in both aqueous and nonaqueous electrolytes. Recent reports have indicated that SERS enhancement factors of up to 14 orders of magnitude can be achieved, providing the sensitivity requisite for ultra trace level detection of target analytes. For this reason, we are developing a method for bacterial endospore SERS detection based on the endospores marker - dipicolinic acid (DPA). The SERS spectra of dipicolinic acid in aqueous solutions are reported. The dipicolinate vibrational features could be observed in the SERS spectra at the concentration as low as 8 × 10 -5 M in 5 minutes. These limits of detection are entirely controlled by the thermodynamics and kinetics of DPA binding to the AgFON surface.",

keywords = "Ag film over nanosphere, Anthrax, Dipicolinic acid, Electrochemistry, Endospore, Surface-enhanced Raman",

author = "Xiaoyu Zhang and Yonzon, {Chanda Ranjit} and {Van Duyne}, {Richard P.}",

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T1 - An electrochemical surface-enhanced Raman spectroscopy approach to anthrax detection

AU - Zhang, Xiaoyu

AU - Yonzon, Chanda Ranjit

AU - Van Duyne, Richard P.

PY - 2003/12/1

Y1 - 2003/12/1

N2 - Metal film over nanosphere (MFON) electrodes are excellent substrates for surface-enhanced Raman scattering (SERS) spectroscopy. These surfaces are produced by vapor deposition of a metal film over nanospheres that are assembled in a hexagonally close packed arrangement. The efficiency and reproducibility of AgFON electrode as SERS substrates are confirmed by the repeatability of the electrochemical surface enhanced Raman scattering spectra of pyridine and the Ru(bpy)3 3+/Ru(bpy)3 2+ complexes adsorbed on AgFON electrodes. The Raman signal for AgFON electrodes is observed to be extremely stable even at extremely negative potentials in both aqueous and nonaqueous electrolytes. Recent reports have indicated that SERS enhancement factors of up to 14 orders of magnitude can be achieved, providing the sensitivity requisite for ultra trace level detection of target analytes. For this reason, we are developing a method for bacterial endospore SERS detection based on the endospores marker - dipicolinic acid (DPA). The SERS spectra of dipicolinic acid in aqueous solutions are reported. The dipicolinate vibrational features could be observed in the SERS spectra at the concentration as low as 8 × 10 -5 M in 5 minutes. These limits of detection are entirely controlled by the thermodynamics and kinetics of DPA binding to the AgFON surface.

AB - Metal film over nanosphere (MFON) electrodes are excellent substrates for surface-enhanced Raman scattering (SERS) spectroscopy. These surfaces are produced by vapor deposition of a metal film over nanospheres that are assembled in a hexagonally close packed arrangement. The efficiency and reproducibility of AgFON electrode as SERS substrates are confirmed by the repeatability of the electrochemical surface enhanced Raman scattering spectra of pyridine and the Ru(bpy)3 3+/Ru(bpy)3 2+ complexes adsorbed on AgFON electrodes. The Raman signal for AgFON electrodes is observed to be extremely stable even at extremely negative potentials in both aqueous and nonaqueous electrolytes. Recent reports have indicated that SERS enhancement factors of up to 14 orders of magnitude can be achieved, providing the sensitivity requisite for ultra trace level detection of target analytes. For this reason, we are developing a method for bacterial endospore SERS detection based on the endospores marker - dipicolinic acid (DPA). The SERS spectra of dipicolinic acid in aqueous solutions are reported. The dipicolinate vibrational features could be observed in the SERS spectra at the concentration as low as 8 × 10 -5 M in 5 minutes. These limits of detection are entirely controlled by the thermodynamics and kinetics of DPA binding to the AgFON surface.

KW - Ag film over nanosphere

KW - Anthrax

KW - Dipicolinic acid

KW - Electrochemistry

KW - Endospore

KW - Surface-enhanced Raman

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Y2 - 3 August 2003 through 5 August 2003

ER -

An electrochemical surface-enhanced Raman spectroscopy approach to anthrax detection

Abstract

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