An ELISA method detecting the active form of suPAR

Xiaolei Zhou, Mingming Xu, Hailong Huang, Andrew Mazar, Zafar Iqbal, Cai Yuan*, Mingdong Huang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Urokinase plasminogen activator receptor (uPAR) exists in a number of formats in human plasma, including soluble uPAR (suPAR) and uPAR fragments. We developed an ELISA method to detect specifically the active form suPAR, which binds to its natural ligand uPA. The intra CV and inter CV of this ELISA assay is 8.5% and 9.6% respectively, and the assay can recover 99.74% of added recombinant suPAR from 10% plasma. This assay is quite sensitive, capable of detecting down to 15 pg/ml of suPAR, and can measure suPAR concentrations in the range of 0.031–8 ng/ml with high linear relationship. Plasma samples from pregnant women were also measured for the active form of suPAR with this assay, giving an averaged level of 1.39 ng/ml, slightly higher than the level of pooled plasma from healthy donors (0.96 ng/ml). This study demonstrates the feasibility to measure the active form of suPAR, which will likely have value in clinical applications.

Original languageEnglish (US)
Pages (from-to)205-210
Number of pages6
JournalTalanta
Volume160
DOIs
StatePublished - Nov 1 2016

Keywords

  • Active suPAR
  • ELISA
  • Pregnant women
  • suPAR

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'An ELISA method detecting the active form of suPAR'. Together they form a unique fingerprint.

Cite this