An endovascular canine middle cerebral artery occlusion model for the study of leptomeningeal collateral recruitment

Gregory A. Christoforidis, Cameron Rink, Marinos S. Kontzialis, Yousef Mohammad, Regina M. Koch, Amir M. Abduljalil, Valerie K. Bergdall, Sashwati Roy, Savita Khanna, Andrew P. Slivka, Michael V. Knopp, Chandan K. Sen

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Objectives: This work aimed to refine a large animal in minimally invasive reversible middle cerebral artery (MCA) occlusion (MCAO) model to account for leptomeningeal collateral formation. Materials and methods: An angiographically based methodology allowed for transient MCA and carotid terminus occlusion in 12 mongrel dogs and assessment of pial collateral recruitment. Outcome measures included 1- and 24-hour magnetic resonance imaging-based infarct volume calculation, a behavioral scale and histopathologic sections. Results: MCAO succeeded in 8 of 12 dogs (67% efficiency). One-hour postreperfusion infarct volume predicted 24-hour postreperfusion infarct volume (r = 0.997, P < 0.0001). Pial collateral recruitment varied with time and reproducibly assessed predicted infarct volume on 1-hour postreperfusion mean diffusivity maps (P < 0.0001; r = 0.946) and 24-hour fluid-attenuated inversion recovery FLAIR magnetic resonance imaging (P = 0.0033; r = 0.961). The canine stroke scale score correlated with infarct volumes and pial collateral score. Conclusion: This canine MCAO model produces defined cerebral infarct lesions whose volumes correlate with leptomeningeal collateral formation and canine behavior.

Original languageEnglish (US)
Pages (from-to)34-40
Number of pages7
JournalInvestigative radiology
Volume46
Issue number1
DOIs
StatePublished - Jan 2011
Externally publishedYes

Keywords

  • angiography
  • animal models
  • collateral circulation
  • diffusion tensor imaging
  • middle cerebral artery infarction
  • stroke assessment

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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