An EXAFS study of zinc coordination in microbial cells

Samuel M. Webb, Jean-Francois Gaillard*, Bradley E. Jackson, David A. Stahl

*Corresponding author for this work

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Five microbes were isolated from metal amended enrichment cultures derived from the sediments of a lake contaminated by a zinc smelter. Each of these organisms was grown in pure culture in the presence of zinc. Quick Extended X-ray Absorption Fine Structure (QEXAFS) spectroscopy was used to investigate the average coordination environment of the zinc associated with the microbial biomass. Fitting of the first coordination shell of zinc shows that significant differences exist for each microbial species examined. The coordination environment of zinc varies between sulfurs to six-fold nitrogen/oxygen, with two microbial strains showing mixed coordination shells. Further study is required in order to characterize these sites and their locations within the cell.

Original languageEnglish (US)
Pages (from-to)943-945
Number of pages3
JournalJournal of Synchrotron Radiation
Volume8
Issue number2
DOIs
StatePublished - Mar 1 2001

Keywords

  • EXAFS
  • Metal coordination by microbes

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)
  • Instrumentation
  • Atomic and Molecular Physics, and Optics
  • Radiation

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