An experimental brainstem tumor model using in vivo bioluminescence imaging in rat

Akihide Kondo; Stewart Goldman; Elio F. Vanin; Simone T. Sredni; Veena Rajaram; Marcelo B. Soares; Tadanori Tomita

doi:10.1007/s00381-008-0783-5

An experimental brainstem tumor model using in vivo bioluminescence imaging in rat

Akihide Kondo^*, Stewart Goldman, Elio F. Vanin, Simone T. Sredni, Veena Rajaram, Marcelo B. Soares, Tadanori Tomita

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Purpose: Currently, there is no conclusive treatment for brainstem tumor. To facilitate the development of new treatments, it is essential to establish predictive preclinical in vivo models in which therapeutic modalities can be evaluated. Although a few rodent models have been reported, there is no novel approach that can monitor tumor response qualitatively and quantitatively. Materials and methods: Bioluminescence imaging was used to characterize a rat brainstem tumor model. In this model, 9L gliosarcoma cells, transduced with an onco-retroviral vector containing the luciferase coding sequence, were inoculated into Fisher 344 rats. Result: Histopathological assessment showed successful cell implantation into the brainstem. There was a strong correlation between pathological tumor volume and luminescence strength. Longitudinal quantitative responses of the tumor after application of a therapeutic agent were also demonstrated. Conclusion: This study demonstrates a robust rodent model with the ability to monitor brainstem tumor growth and response to chemotherapeutic agents.

Original language	English (US)
Pages (from-to)	527-533
Number of pages	7
Journal	Child's Nervous System
Volume	25
Issue number	5
DOIs	https://doi.org/10.1007/s00381-008-0783-5
State	Published - May 2009

Funding

Acknowledgments This project was supported by the Rory David Deutsch Foundation, the Neuro-Oncology Research Foundation of Children’s Memorial Hospital, Chicago, IL, USA and the Dr. Ralph and Marian C. Falk Medical Research Trust, Chicago, IL, USA. We thank Christopher A. Hamm for his cooperation with the transduction procedure. We also acknowledge the Department of Pathology and Laboratory Medicine, Children’s Memorial Hospital, Chicago, IL, USA, Ms. Kelly Arndt, Merrill J. Egorin, M.D., Barbara Mania-Farnell, Ph.D., and all the members of the Soares Laboratory (Cancer Biology and Epigenomics Program, Children’s Memorial Research Center) for their critical reading of this manuscript and technical advice.

Keywords

Bioluminescence
Brainstem glioma
Chemotherapy
Experimental rodent model
In vivo imaging

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health
Clinical Neurology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00381-008-0783-5

Cite this

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title = "An experimental brainstem tumor model using in vivo bioluminescence imaging in rat",

abstract = "Purpose: Currently, there is no conclusive treatment for brainstem tumor. To facilitate the development of new treatments, it is essential to establish predictive preclinical in vivo models in which therapeutic modalities can be evaluated. Although a few rodent models have been reported, there is no novel approach that can monitor tumor response qualitatively and quantitatively. Materials and methods: Bioluminescence imaging was used to characterize a rat brainstem tumor model. In this model, 9L gliosarcoma cells, transduced with an onco-retroviral vector containing the luciferase coding sequence, were inoculated into Fisher 344 rats. Result: Histopathological assessment showed successful cell implantation into the brainstem. There was a strong correlation between pathological tumor volume and luminescence strength. Longitudinal quantitative responses of the tumor after application of a therapeutic agent were also demonstrated. Conclusion: This study demonstrates a robust rodent model with the ability to monitor brainstem tumor growth and response to chemotherapeutic agents.",

keywords = "Bioluminescence, Brainstem glioma, Chemotherapy, Experimental rodent model, In vivo imaging",

author = "Akihide Kondo and Stewart Goldman and Vanin, {Elio F.} and Sredni, {Simone T.} and Veena Rajaram and Soares, {Marcelo B.} and Tadanori Tomita",

note = "Funding Information: Acknowledgments This project was supported by the Rory David Deutsch Foundation, the Neuro-Oncology Research Foundation of Children{\textquoteright}s Memorial Hospital, Chicago, IL, USA and the Dr. Ralph and Marian C. Falk Medical Research Trust, Chicago, IL, USA. We thank Christopher A. Hamm for his cooperation with the transduction procedure. We also acknowledge the Department of Pathology and Laboratory Medicine, Children{\textquoteright}s Memorial Hospital, Chicago, IL, USA, Ms. Kelly Arndt, Merrill J. Egorin, M.D., Barbara Mania-Farnell, Ph.D., and all the members of the Soares Laboratory (Cancer Biology and Epigenomics Program, Children{\textquoteright}s Memorial Research Center) for their critical reading of this manuscript and technical advice. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

year = "2009",

month = may,

doi = "10.1007/s00381-008-0783-5",

language = "English (US)",

volume = "25",

pages = "527--533",

journal = "Child's Nervous System",

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AU - Kondo, Akihide

AU - Goldman, Stewart

AU - Vanin, Elio F.

AU - Sredni, Simone T.

AU - Rajaram, Veena

AU - Soares, Marcelo B.

AU - Tomita, Tadanori

N1 - Funding Information: Acknowledgments This project was supported by the Rory David Deutsch Foundation, the Neuro-Oncology Research Foundation of Children’s Memorial Hospital, Chicago, IL, USA and the Dr. Ralph and Marian C. Falk Medical Research Trust, Chicago, IL, USA. We thank Christopher A. Hamm for his cooperation with the transduction procedure. We also acknowledge the Department of Pathology and Laboratory Medicine, Children’s Memorial Hospital, Chicago, IL, USA, Ms. Kelly Arndt, Merrill J. Egorin, M.D., Barbara Mania-Farnell, Ph.D., and all the members of the Soares Laboratory (Cancer Biology and Epigenomics Program, Children’s Memorial Research Center) for their critical reading of this manuscript and technical advice. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

PY - 2009/5

Y1 - 2009/5

N2 - Purpose: Currently, there is no conclusive treatment for brainstem tumor. To facilitate the development of new treatments, it is essential to establish predictive preclinical in vivo models in which therapeutic modalities can be evaluated. Although a few rodent models have been reported, there is no novel approach that can monitor tumor response qualitatively and quantitatively. Materials and methods: Bioluminescence imaging was used to characterize a rat brainstem tumor model. In this model, 9L gliosarcoma cells, transduced with an onco-retroviral vector containing the luciferase coding sequence, were inoculated into Fisher 344 rats. Result: Histopathological assessment showed successful cell implantation into the brainstem. There was a strong correlation between pathological tumor volume and luminescence strength. Longitudinal quantitative responses of the tumor after application of a therapeutic agent were also demonstrated. Conclusion: This study demonstrates a robust rodent model with the ability to monitor brainstem tumor growth and response to chemotherapeutic agents.

AB - Purpose: Currently, there is no conclusive treatment for brainstem tumor. To facilitate the development of new treatments, it is essential to establish predictive preclinical in vivo models in which therapeutic modalities can be evaluated. Although a few rodent models have been reported, there is no novel approach that can monitor tumor response qualitatively and quantitatively. Materials and methods: Bioluminescence imaging was used to characterize a rat brainstem tumor model. In this model, 9L gliosarcoma cells, transduced with an onco-retroviral vector containing the luciferase coding sequence, were inoculated into Fisher 344 rats. Result: Histopathological assessment showed successful cell implantation into the brainstem. There was a strong correlation between pathological tumor volume and luminescence strength. Longitudinal quantitative responses of the tumor after application of a therapeutic agent were also demonstrated. Conclusion: This study demonstrates a robust rodent model with the ability to monitor brainstem tumor growth and response to chemotherapeutic agents.

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An experimental brainstem tumor model using in vivo bioluminescence imaging in rat

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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