An experimental model of intraabdominal abscess in the rat.

D. E. Fry*, R. N. Garrison, R. D. Rink, J. Casey, M. M. DeCamp, J. D. Richardson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Suitable small animal models of intraabdominal abscess are not available. We have developed a reliable model of intraabdominal abscess in the rat by fasting the animal overnight, devascularizing and ligating the cecum, and injecting 2.5 X 10(8) Escherichia coli and 2.5 X 10(9) Bacteroides fragilis into the ligated cecum. This model results in 39% coli acute mortalities within 48 hours from acute peritonitis, and abscess in 76% of survivals. Subsequent abscesses are 1.0--1.5 ml in volume and contain 4 X 10(8) E coli/ml of pus and 1.4 X 10(9) B fragilis/ml. This model allows chronic evaluation of sepsis in small animals and provides a convenient means to study the abscess microenvironment.

Original languageEnglish (US)
Pages (from-to)7-11
Number of pages5
JournalAdvances in shock research
Volume7
StatePublished - Jan 1 1982

ASJC Scopus subject areas

  • Medicine(all)

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