An Immobilized Enzyme Reactor for Spatiotemporal Control over Reaction Products

Jennifer Grant, Justin A. Modica, Juliet Roll, Paul Perkovich, Milan Mrksich*

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

This paper describes a microfluidic chip wherein the position and order of two immobilized enzymes affects the type and quantity of reaction products in the flowing fluid. Assembly of the chip is based on a self-assembled monolayer presenting two orthogonal covalent capture ligands that immobilize their respective fusion enzyme. A thiol-tagged substrate is flowed over a region presenting the first enzyme—which generates a product that is efficiently transferred to the second enzyme—and the second enzyme's product binds to an adjacent thiol capture site on the chip. The amount of the three possible reaction products is quantified directly on the chip using self-assembled monolayers for matrix-assisted laser desorption/ionization mass spectrometry, revealing that the same microsystem can be spatiotemporally arranged to produce different products depending on the device design. This work allows for optimizing multistep biochemical transformations in favor of a desired product using a facile reaction and analytical format.

Original languageEnglish (US)
Article number1800923
JournalSmall
Volume14
Issue number31
DOIs
StatePublished - Aug 2 2018

Keywords

  • MALDI mass spectrometry
  • immobilized enzymes
  • microfluidics
  • microreactors
  • self-assembled monolayers

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

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