An immunocytochemical study of the routes of secretion of collagen and phosphophoryn from odontoblasts into dentin

A. M. Rabie*, Arthur Veis

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Polyclonal antibodies to rat incisor phosphophoryns and to the amino-telopeptide of the α (I)-chain of type I collagen were used to follow the pathways of movement of collagen 1 (COL 1) and phosphophoryns (PP) from synthesis in the odontoblast to secretion into the mineralized dentin. The antibodies were detected at the transmission electron microscopic level by their reaction with Protein A-colloidal gold conjugates. Special care was given in specimen preparation to retention of maximal antigenicity during fixation while maintaining cellular and extracellular ultrastructure at the mineralization front (MF) in nondemineralized sections. Intracellularly, COL1 and PP were detected within the endoplasmic reticulum (ER), the Golgi (G) and secretory granules (SO). However, as determined by double-immunolabeling with different size gold particles the COL1 and PP were not found together within the same ER, G or SG compartments. PP was localized within the tubular ER, round-shaped transitional vesicles, the Golgi and in narrow asymmetric SG. These asymmetric SG were found in abundance in the odontoblastic process. PP secretion from these vesicles was near the MF at the predentin-dentin boundary. COL1 was localized within rosette form ER compartments, the Golgi and in large, distinctive SG. COL 1 was deposited at the cell-predentin boundary. No COL 1 SG were seen within the odontoblastic process near the MF. In the region of the MF, prior to mineralization, the PP was localized along the surfaces of the COL1 fibrils of the predentin. The mineral phase etched surfaces revealed both COL 1 - and abundant mineral-associated PP. These data support the hypotheses that, in dentin, the interaction between COL I and PP may initiate crystal nucleation and that additional interactions between PP and the growing crystals may modulate the crystal growth pattern and crystal size.

Original languageEnglish (US)
Pages (from-to)197-209
Number of pages13
JournalConnective tissue research
Issue number3
StatePublished - 1995


  • Collagen
  • Dentin
  • Intracellular transport
  • Mineralization
  • Phosphophoryn
  • Secretory pathways

ASJC Scopus subject areas

  • Rheumatology
  • Biochemistry
  • Orthopedics and Sports Medicine
  • Molecular Biology
  • Cell Biology


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