An Improved SDS–Polyacrylamide Gel Electrophoresis for Resolution of Peptides in the Range of 3.5–200KDA

Zhila Khalkhali-Ellis

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Resolution of a wide range of polypeptides, 3.5-200kDa, on a single low acrylamide and cross linkage gel of 7.7% T, 2.6% C is described here. Laemmli’s (4) original discontinuous SDS polyacrylamide gel electrophoresis (SDS-PAGE) system is modified by increasing the ionic strength of both stacking and resolving gels, and replacing the usual glycine buffer with a tricine cathode buffer as described by Schagger and von Jagow (7). This system offers the advantage of a wide range of protein fractionation, with sufficient band resolution, on a single, low acrylamide concentration and cross 1inkage gel. Moreover, increased gel ionic concentration allows higher protein and salt load, and renders this system suitable for preparative work.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalPreparative Biochemistry
Volume25
Issue number1-2
DOIs
StatePublished - 1995

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

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