An in vitro demonstration of peroxisome proliferation and increase in peroxisomal β-oxidation system mRNAs in cultured rat hepatocytes treated with ciprofibrate

Shobha Thangada*, Keith Alvares, Mario Mangino, Mohammed I. Usman, M. Sambasiva Rao, Janardan K. Reddy

*Corresponding author for this work

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Using the normal adult rat hepatocytes, plated on rat tail collagen-coated dishes and fed a chemically defined medium, we demonstrate here that ciprofibrate at 0.1 mM concentration, increases significantly the mRNA levels of fatty acyl-CoA oxidase, enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase bifunctional protein, and thiolase (the three enzymes of the β-oxidation system), and causes peroxisome proliferation. Increase in mRNA levels of these genes was evident within 1 h and was maximal 24 h after the addition of ciprofibrate. In hepatocytes cultured in the absence of ciprofibrate, the basal levels of these enzymes were low and further declined with time. Concomitant treatment of hepatocytes with cycloheximide did not inhibit or superinduce the mRNA levels, indicating that this induction may represent a primary (direct) effect of this compound on the expression of these genes and does not apparently involve short-lived repressor protein(s).

Original languageEnglish (US)
Pages (from-to)205-210
Number of pages6
JournalFEBS Letters
Volume250
Issue number2
DOIs
StatePublished - Jul 3 1989

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Keywords

  • (Rat hepatocyte)
  • Peroxisome proliferation
  • Tissue culture
  • mRNA

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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