An open-access microfluidic model for lung-specific functional studies at an air-liquid interface

Divya D. Nalayanda, Christopher Puleo, William B. Fulton, Leilani M. Sharpe, Tza Huei Wang, Fizan Abdullah*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

In an effort to improve the physiologic relevance of existing in vitro models for alveolar cells, we present a microfluidic platform which provides an air-interface in a dynamic system combining microfluidic and suspended membrane culture systems. Such a system provides the ability to manipulate multiple parameters on a single platform along with ease in cell seeding and manipulation. The current study presents a comparison of the efficacy of the hybrid system with conventional platforms using assays analyzing the maintenance of function and integrity of A549 alveolar epithelial cell monolayer cultures. The hybrid system incorporates bio-mimetic nourishment on the basal side of the epithelial cells along with an open system on the apical side of the cells exposed to air allowing for easy access for assays.

Original languageEnglish (US)
Pages (from-to)1081-1089
Number of pages9
JournalBiomedical Microdevices
Volume11
Issue number5
DOIs
StatePublished - Oct 1 2009

Keywords

  • Air-liquid interface
  • Alveolar cells
  • Microfluidic cell culture

ASJC Scopus subject areas

  • Biomedical Engineering
  • Molecular Biology

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