An optimized protocol for isolating primary epithelial cell chromatin for ChIP

Keyphrases

• Chromatin Immunoprecipitation (ChIP) 100%
• Chromatin 100%
• Primary Epithelial Cells 100%
• Protocol Optimization 100%
• Formaldehyde 50%
• Membrane Lysis 50%
• Lysis Buffer 50%
• Cell Membrane 33%
• Fixed Cells 33%
• Lysis 33%
• Size Selectivity 33%
• Cell Suspension 16%
• Room Temperature 16%
• Next-generation Sequencing 16%
• Epithelial Cells 16%
• Modified Technique 16%
• Light Microscopy 16%
• DNA-binding Proteins 16%
• Trypsin 16%
• High Molecular Weight 16%
• Incubation 16%
• Agitation 16%
• Cell Lysis 16%
• Equilibration 16%
• Triton X-100 (TX-100) 16%
• Adherent Cells 16%
• Modified Protocol 16%
• Primary Bronchial Epithelial Cells 16%
• Methyl Green 16%
• Pyronin 16%
• Dual Dye 16%
• Detergent-free 16%
• Cross-linked Materials 16%
• Methyl Green Pyronin 16%
• 25-Gauge Needle 16%

Immunology and Microbiology

• Chromatin Immunoprecipitation 100%
• Cell Membrane 33%
• Light Microscopy 16%
• Next Generation Sequencing 16%
• Molecular Weight 16%
• Ultrasound 16%
• Cytolysis 16%
• Room Temperature 16%
• DNA Binding Protein 16%
• Cell Suspension 16%
• Adherent Cell 16%
• Ethylenediaminetetraacetic Acid 16%

Biochemistry, Genetics and Molecular Biology

• Chromatin Immunoprecipitation 100%
• Cell Membrane 33%
• Ultrasound 16%
• Copurification 16%
• DNA-binding Protein 16%
• Next Generation Sequencing 16%
• Molecular Weight 16%
• Room Temperature 16%
• Cell Suspension 16%
• Cytolysis 16%