An ultraviolet light-induced crosslink in yeast tRNA

Linda S. Behlen; Jeffrey R. Sampson; Olke C. Uhlenbeck

doi:10.1093/nar/20.15.4055

An ultraviolet light-induced crosslink in yeast tRNA

Linda S. Behlen, Jeffrey R. Sampson, Olke C. Uhlenbeck^*

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

The irradiation of native or unmodified yeast tRNA^phe by short wavelength UV light (260 nM) results In an Intramolecular crosslink that has been mapped to occur between C48 In the variable loop and U59 in the T loop. Photo-reversibility of the crosslink and the absence of fluorescent photo adducts suggest that the crosslink product is a cytidine-uridine cyclobutane dimer. This Is consistent with the relative geometries of C48 and U59 in the crystal structure of yeast tRNA^phe. Evaluation of the crosslinking efficiency of the mutants of tRNA^Phe indicates that the reaction depends on the correct tertiary structure of the RNA.

Original language	English (US)
Pages (from-to)	4055-4059
Number of pages	5
Journal	Nucleic acids research
Volume	20
Issue number	15
DOIs	https://doi.org/10.1093/nar/20.15.4055
State	Published - Aug 11 1992

ASJC Scopus subject areas

Genetics

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title = "An ultraviolet light-induced crosslink in yeast tRNA",

abstract = "The irradiation of native or unmodified yeast tRNAphe by short wavelength UV light (260 nM) results In an Intramolecular crosslink that has been mapped to occur between C48 In the variable loop and U59 in the T loop. Photo-reversibility of the crosslink and the absence of fluorescent photo adducts suggest that the crosslink product is a cytidine-uridine cyclobutane dimer. This Is consistent with the relative geometries of C48 and U59 in the crystal structure of yeast tRNAphe. Evaluation of the crosslinking efficiency of the mutants of tRNAPhe indicates that the reaction depends on the correct tertiary structure of the RNA.",

author = "Behlen, {Linda S.} and Sampson, {Jeffrey R.} and Uhlenbeck, {Olke C.}",

note = "Funding Information: We would like to thank Ellen Tinkle Peterson for useful discussions and Fig. 3b. This research has been supported by a National Institutes of Health Grant GM37552. We also thank the W.M.Keck Foundation for their generous support of RNA science on the Boulder campus.",

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T1 - An ultraviolet light-induced crosslink in yeast tRNA

AU - Behlen, Linda S.

AU - Sampson, Jeffrey R.

AU - Uhlenbeck, Olke C.

N1 - Funding Information: We would like to thank Ellen Tinkle Peterson for useful discussions and Fig. 3b. This research has been supported by a National Institutes of Health Grant GM37552. We also thank the W.M.Keck Foundation for their generous support of RNA science on the Boulder campus.

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Y1 - 1992/8/11

N2 - The irradiation of native or unmodified yeast tRNAphe by short wavelength UV light (260 nM) results In an Intramolecular crosslink that has been mapped to occur between C48 In the variable loop and U59 in the T loop. Photo-reversibility of the crosslink and the absence of fluorescent photo adducts suggest that the crosslink product is a cytidine-uridine cyclobutane dimer. This Is consistent with the relative geometries of C48 and U59 in the crystal structure of yeast tRNAphe. Evaluation of the crosslinking efficiency of the mutants of tRNAPhe indicates that the reaction depends on the correct tertiary structure of the RNA.

AB - The irradiation of native or unmodified yeast tRNAphe by short wavelength UV light (260 nM) results In an Intramolecular crosslink that has been mapped to occur between C48 In the variable loop and U59 in the T loop. Photo-reversibility of the crosslink and the absence of fluorescent photo adducts suggest that the crosslink product is a cytidine-uridine cyclobutane dimer. This Is consistent with the relative geometries of C48 and U59 in the crystal structure of yeast tRNAphe. Evaluation of the crosslinking efficiency of the mutants of tRNAPhe indicates that the reaction depends on the correct tertiary structure of the RNA.

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