An updated protocol for the cost-effective and weekend-free culture of human induced pluripotent stem cells

Davi Marco Lyra-Leite; Hananeh Fonoudi; Mennat Gharib; Paul W. Burridge

doi:10.1016/j.xpro.2020.100213

An updated protocol for the cost-effective and weekend-free culture of human induced pluripotent stem cells

Davi Marco Lyra-Leite, Hananeh Fonoudi, Mennat Gharib, Paul W. Burridge^*

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The protocol provided here describes methodologies for making a highly cost-effective, chemically defined medium for culturing hiPSCs we call B8 medium. The typical cost of B8 medium is US$10 per liter, which with modifications included here is more affordable than standard media. We provide simple protocols for making B8 supplement aliquots, making the basal media DMEM/F12, Matrigel-coated plates, thawing, passaging, culturing, and cryopreserving hiPSCs. We show typical differentiation results and provide a comprehensive troubleshooting guide. For complete details on the use and execution of this protocol, please refer to Kuo et al. (2020).

Original language	English (US)
Article number	100213
Journal	STAR Protocols
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2020.100213
State	Published - Mar 19 2021

Keywords

Cell Culture
Stem Cells

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Neuroscience
General Immunology and Microbiology
General Medicine

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10.1016/j.xpro.2020.100213

Cite this

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abstract = "The protocol provided here describes methodologies for making a highly cost-effective, chemically defined medium for culturing hiPSCs we call B8 medium. The typical cost of B8 medium is US$10 per liter, which with modifications included here is more affordable than standard media. We provide simple protocols for making B8 supplement aliquots, making the basal media DMEM/F12, Matrigel-coated plates, thawing, passaging, culturing, and cryopreserving hiPSCs. We show typical differentiation results and provide a comprehensive troubleshooting guide. For complete details on the use and execution of this protocol, please refer to Kuo et al. (2020).",

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AU - Burridge, Paul W.

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An updated protocol for the cost-effective and weekend-free culture of human induced pluripotent stem cells

Abstract

Keywords

ASJC Scopus subject areas

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