An updated protocol for the cost-effective and weekend-free culture of human induced pluripotent stem cells

Davi Marco Lyra-Leite, Hananeh Fonoudi, Mennat Gharib, Paul W. Burridge*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The protocol provided here describes methodologies for making a highly cost-effective, chemically defined medium for culturing hiPSCs we call B8 medium. The typical cost of B8 medium is US$10 per liter, which with modifications included here is more affordable than standard media. We provide simple protocols for making B8 supplement aliquots, making the basal media DMEM/F12, Matrigel-coated plates, thawing, passaging, culturing, and cryopreserving hiPSCs. We show typical differentiation results and provide a comprehensive troubleshooting guide. For complete details on the use and execution of this protocol, please refer to Kuo et al. (2020).

Original languageEnglish (US)
Article number100213
JournalSTAR Protocols
Volume2
Issue number1
DOIs
StatePublished - Mar 19 2021

Keywords

  • Cell Culture
  • Stem Cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)

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