Analyses of the dynamic properties of nuclear lamins by fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy (FCS)

Shimi Takeshi, Chan Gi Pack, Robert D. Goldman

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

The major structural components of the nuclear lamina are the A- and B-type nuclear lamin proteins which are also present in the nucleoplasm. Studies of molecular movements of the lamins in both the lamina and nucleoplasm of living cell nuclei have provided insights into their roles in maintaining nuclear architecture. In this chapter, we present protocols for quantitatively measuring the mobilities of lamin proteins by fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy (FCS) in mammalian cell nuclei.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages99-111
Number of pages13
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1411
ISSN (Print)1064-3745

Keywords

  • FCS
  • FRAP
  • Lamin
  • Lamina

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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