Analysis and characterization of antiviral drug-resistant cytomegalovirus isolates from solid organ transplant recipients

Nell S. Lurain, Sangeeta M. Bhorade, Kenneth J. Pursell, Robin K. Avery, Vijay V. Yeldandi, Carlos M. Isada, Emmanuel S. Robert, Debra J. Kohn, Max Q. Arens, Edward R. Garrity, Alan J. Taege, Martin G. Mullen, Kathleen M. Todd, James W. Bremer, Belinda Yen-Lieberman

Research output: Contribution to journalArticlepeer-review

98 Scopus citations

Abstract

The development of cytomegalovirus (CMV) disease and subsequent emergence of drug-resistant strains was examined in a large group of solid organ transplant recipients; drug-resistant CMV was detected in a total of 30 transplant recipients (20 lung, 5 kidney, 4 heart, and 1 liver). Drug resistance was confirmed both phenotypically and genotypically. The sequences of drug-resistant CMV strains from the same patient differed from drug-susceptible baseline sequences only at single sites previously confirmed to confer drug resistance. At least 1 isolate from each patient had a mutation in the UL97 phosphotransferase coding sequence. Mutations in the DNA polymerase gene were found in 6 of 38 sequenced strains. Lung transplant recipients had the highest incidence of drug-resistant virus: of the 30 patients, 28 were CMV-seronegative transplant recipients of CMV-seropositive organs, which strongly supports the premise that drug resistance is most prevalent in that transplant population.

Original languageEnglish (US)
Pages (from-to)760-768
Number of pages9
JournalJournal of Infectious Diseases
Volume186
Issue number6
DOIs
StatePublished - Sep 15 2002

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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