Analysis by array CGH of genomic changes associated with the progression or relapse of Wilm's tumour

R. Natrajan, S. E. Little, N. Sodha, J. S. Reis-Filho, A. Mackay, K. Fenwick, A. Ashworth, E. J. Perlman, J. S. Dome, P. E. Grundy, K. Pritchard-Jones, Chris Jones*

*Corresponding author for this work

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

Despite aggressive salvage regimens, approximately half of all children who suffer a Wilms' tumour recurrence will die of their disease. Although there are increasing data on molecular genetic prognostic factors present in the tumour at diagnosis, there is little information regarding the molecular events that occur with Wilms' tumour progression and relapse. In the present study, microarray-based comparative genomic hybridization (aCGH) analysis has been carried out on 58 Wilms' tumour samples, which included 38 untreated primary and 20 recurrent tumours. A higher degree of copy number changes was observed in the recurrent tumours (33.0% genomic clones) than in the primary tumour (21.2%). Paired analysis highlighted the acquisition of 15q gain with high levels of IGFIR expression in the tumour recurrence in two cases. The most statistically significant abnormality acquired between diagnosis and relapse was loss of 17p. One case that experienced 17p loss was classified as favourable histology at diagnosis, but exhibited diffuse anaplasia at recurrence and had a homozygous TP53 deletion. Another instructive case with a constitutional 11p13 deletion presented with bilateral tumours and suffered two subsequent recurrences in the left kidney. A somatic WT1 mutation was found only in the right kidney tumour, while the constitutional 11p13 deletion was the only abnormality detected in the initial left kidney tumour by aCGH. The two subsequent relapses in the left kidney contained an accumulation of additional genetic alterations, including an independent WT1 mutation.

Original languageEnglish (US)
Pages (from-to)52-59
Number of pages8
JournalJournal of Pathology
Volume211
Issue number1
DOIs
StatePublished - Jan 2007

Keywords

  • 17p
  • Anaplasia
  • Relapse
  • TP53
  • WT1
  • Wilms' tumour
  • aCGH

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Analysis by array CGH of genomic changes associated with the progression or relapse of Wilm's tumour'. Together they form a unique fingerprint.

  • Cite this

    Natrajan, R., Little, S. E., Sodha, N., Reis-Filho, J. S., Mackay, A., Fenwick, K., Ashworth, A., Perlman, E. J., Dome, J. S., Grundy, P. E., Pritchard-Jones, K., & Jones, C. (2007). Analysis by array CGH of genomic changes associated with the progression or relapse of Wilm's tumour. Journal of Pathology, 211(1), 52-59. https://doi.org/10.1002/path.2087