Analysis of cellular localization and function of carboxy-terminal mutants of pendrin

Aigerim Bizhanova*, Teng Leong Chew, Satya Khuon, Peter Kopp

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Background: Iodide uptake at the basolateral membrane and iodide efflux at the apical membrane of thyrocytes, essential steps in the biosynthesis of thyroid hormone, are stimulated by thyroid stimulating hormone (TSH). Pendrin (SLC26A4) is inserted into the apical membrane of thyrocytes and thought to be involved in mediating iodide efflux. Methods: We determined the effects of carboxy-terminal mutations of pendrin on the cellular localization and the ability to transport iodide. Results: After exposure to forskolin, the membrane abundance of wild type pendrin and iodide efflux increase. Truncation mutants lead to complete intracellular retention. Elimination of the distal part of the sulfate transporter and antisigma factor antagonist (STAS) domain with retention of the putative protein kinase A (PKA) phosphorylation site (RKDT 714-717) results in residual membrane insertion and a partial loss of function. Deletion of the PKA site results in decreased basal function and membrane insertion and abolishes the response to forskolin. Conclusion: Pendrin membrane abundance and its ability to mediate iodide efflux increase after activation of the PKA pathway. Elimination of the PKA site abolishes the response to forskolin but partial basal function and membrane insertion are maintained.

Original languageEnglish (US)
Pages (from-to)423-434
Number of pages12
JournalCellular Physiology and Biochemistry
Volume28
Issue number3
DOIs
StatePublished - 2011

Keywords

  • Iodide transport
  • Mutation
  • Pendrin
  • SLC26A4
  • Thyroid hormone synthesis

ASJC Scopus subject areas

  • Physiology

Fingerprint Dive into the research topics of 'Analysis of cellular localization and function of carboxy-terminal mutants of pendrin'. Together they form a unique fingerprint.

Cite this