Abstract
The frequency of numerical and structural chromosomal abnormalities was studied in the sperm of a lymphoma patient 3 years after MACOP-B chemotherapy (CT). Sperm karyotyping was performed by fusion of human sperm with hamster oocytes and analysis of 193 Q-banded sperm chromosomes. Multicolor fluorescence in situ hybridization (FISH) was performed on 10,228 sperm for analysis of disomy frequencies for chromosomes 1 and 12 and on 10,664 sperm for chromosomes X and Y. Sperm karyotyping demonstrated numerical abnormalities in 7.3% of the spreads, 6.7% hypohaploid and 0.5% hyperhaploid, giving a conservative estimate of aneuploidy of 1%. Structural chromosomal abnormalities were present in 7.3% of the karyotypes and 0.5% had both numerical and structural abnormalities. Results of FISH analyses yielded disomy frequencies of 0.10%, 0.11%, 0.04%, 0.05%, and 0.18% for chromosomes 1, 12, X, Y, and XY, respectively. The frequency of diploid sperm was 0.09%. The frequency of abnormalities was not significantly increased compared to control donors for any of the studies. Also, the frequencies of X- and Y-bearing sperm did not differ significantly from 50% in the sperm karyotyping or FISH studies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 29-32 |
| Number of pages | 4 |
| Journal | Cancer Genetics and Cytogenetics |
| Volume | 80 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 1995 |
Funding
The authors are grateful for the cooperation of the cancer patient who made this study possible. The expert technical assistance of Leona Barclay, Kathy Hildebrand, and Evelyn Ko, and the secretarial skills of Debbie Bell, are gratefully acknowledged. This research was supported by the Medical Research Council of Canada, the Alberta Heritage Fund for Medical Research, and the Alberta Children's Hospital Research Foundation.
ASJC Scopus subject areas
- Genetics
- Molecular Biology
- Cancer Research
