Analysis of differences between Coomassie blue stain and silver stain procedures in polyacrylamide gels: Conditions for the detection of calmodulin and troponin C

Michael Schleicher*, D. Martin Watterson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

It is reported that the conditions used in some silver stain procedures can fail to detect calmodulin, troponin C, and other proteins with similar physical properties. Conditions are described that allow the reproducible detection of these proteins. Two phenomena are described: (1) lack of protein staining when treatment with glutaraldehyde is omitted from the protocol, and (2) loss of small proteins from the gel matrix during prolonged washing procedures. These data directly demonstrate that the use of some silver staining protocols can result in misleading data in biological studies and provide an explanation for at least one class of proteins of how silver staining and Coomassie blue staining of gels can give different results.

Original languageEnglish (US)
Pages (from-to)312-317
Number of pages6
JournalAnalytical Biochemistry
Volume131
Issue number2
DOIs
StatePublished - Jun 1983

Keywords

  • Coomassie blue stain
  • calcium-binding proteins
  • calmodulin
  • electrophoresis
  • silver stain
  • troponin C

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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