Analysis of DNA ploidy and proliferative activity in relation to histology and N-myc amplification in neuroblastoma

S. L. Cohn, A. W. Rademaker, H. R. Salwen, W. A. Franklin, F. Gonzales-Crussi, S. T. Rosen, K. D. Bauer

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

Diploid DNA content, advanced stage, unfavorable histology, and N-myc amplification are all associated with aggressive disease and poor prognosis in childhood neuroblastoma. DNA diploidy is associated with advanced stage and unfavorable histology, but the relationships among ploidy, N-myc amplification, and proliferative activity are not known. To determine if DNA diploidy is associated with N-myc amplification, we studied 29 neuroblastomas with flow cytometric analysis and Southern blot analysis. Clinical and histologic features were also evaluated. Sixty percent of the N-myc amplified tumors were diploid, compared to 26% of the neuroblastomas, which lacked N-myc amplification (P = 0.11). In our analysis of proliferative activity and N-myc amplification, a higher mean percentage of cells in S phase was seen in the N-myc-amplified tumors (13.4%) than in the unamplified tumors (10%), but again the result was not statistically significant (P = 0.14). Significant associations were seen between unfavourable histology and DNA diploidy (P = 0.05), and between unfavorable histology and high proliferative activity (P = 0.007). Our data suggest that biologic factors other than N-myc amplification play a role in determining the aggressiveness of at least some diploid neuroblastomas.

Original languageEnglish (US)
Pages (from-to)1043-1052
Number of pages10
JournalAmerican Journal of Pathology
Volume136
Issue number5
StatePublished - Jan 1 1990

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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