Analysis of iron requirements and siderophore production

Denise M. Burnside, Nicholas P. Cianciotto*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter


This chapter describes the methods for inducing, detecting, and purifying the Legionella pneumophila siderophore. The first protocol details the methods by which L. pneumophila is cultured to facilitate production of the siderophore, rhizoferrin. This chapter then describes how to purify siderophore from culture supernatants through sequential reversed-phase/weak-anion exchange chromatography and high-performance liquid chromatography. The next section describes assays which allow the detection of the iron-binding capability and the biological activity of the purified siderophore. Lastly, this chapter describes the growth of L. pneumophila in chemically defined liquid medium (CDM) containing various iron sources as a method to assess the iron requirements of L. pneumophila.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages17
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • BCYE
  • BYE
  • Bioassay
  • CAS assay
  • CDM
  • HPLC
  • Iron
  • Iron salts
  • Legionella pneumophila
  • Rhizoferrin
  • Siderophore

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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