Analysis of iron requirements and siderophore production

Denise M. Burnside, Nicholas P Cianciotto

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This chapter describes the methods for inducing, detecting, and purifying the Legionella pneumophila siderophore. The first protocol details the methods by which L. pneumophila is cultured to facilitate production of the siderophore, rhizoferrin. This chapter then describes how to purify siderophore from culture supernatants through sequential reversed-phase/weak-anion exchange chromatography and high-performance liquid chromatography. The next section describes assays which allow the detection of the iron-binding capability and the biological activity of the purified siderophore. Lastly, this chapter describes the growth of L. pneumophila in chemically defined liquid medium (CDM) containing various iron sources as a method to assess the iron requirements of L. pneumophila.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc
Pages3-19
Number of pages17
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1921
ISSN (Print)1064-3745

Fingerprint

Siderophores
Legionella pneumophila
Iron
Anions
Chromatography
High Pressure Liquid Chromatography
Growth

Keywords

  • BCYE
  • BYE
  • Bioassay
  • CAS assay
  • CDM
  • HPLC
  • Iron
  • Iron salts
  • Legionella pneumophila
  • Rhizoferrin
  • Siderophore

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Burnside, D. M., & Cianciotto, N. P. (2019). Analysis of iron requirements and siderophore production. In Methods in Molecular Biology (pp. 3-19). (Methods in Molecular Biology; Vol. 1921). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9048-1_1
Burnside, Denise M. ; Cianciotto, Nicholas P. / Analysis of iron requirements and siderophore production. Methods in Molecular Biology. Humana Press Inc, 2019. pp. 3-19 (Methods in Molecular Biology).
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Burnside, DM & Cianciotto, NP 2019, Analysis of iron requirements and siderophore production. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1921, Humana Press Inc, pp. 3-19. https://doi.org/10.1007/978-1-4939-9048-1_1

Analysis of iron requirements and siderophore production. / Burnside, Denise M.; Cianciotto, Nicholas P.

Methods in Molecular Biology. Humana Press Inc, 2019. p. 3-19 (Methods in Molecular Biology; Vol. 1921).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Burnside DM, Cianciotto NP. Analysis of iron requirements and siderophore production. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 3-19. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9048-1_1