Analysis of small critical regions of Swi1 conferring prion formation, maintenance, and transmission

Stephanie Valtierra, Zhiqiang Du*, Liming Li

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Saccharomyces cerevisiae contains several prion elements, which are epigenetically transmitted as self-perpetuating protein conformations. One such prion is [SWI+], whose protein determinant is Swi1, a subunit of the SWI/SNF chromatinremodeling complex. We previously reported that [SWI+] formation results in a partial loss-of-function phenotype of poor growth in nonglucose medium and abolishment of multicellular features. We also showed that the first 38 amino acids of Swi1 propagated [SWI+]. We show here that a region as small as the first 32 amino acids of Swi1 (Swi11-32) can decorate [SWI+] aggregation and stably maintain and transmit [SWI+] independently of full-length Swi1. Regions smaller than Swi11-32 are either incapable of aggregation or unstably propagate [SWI+]. When fused to Sup35MC, the [PSI+] determinant lacking its PrD, Swi11-31 and Swi11-32 can act as transferable prion domains (PrDs). The resulting fusions give rise to a novel chimeric prion, [SPS+], exhibiting [PSI+]-like nonsense suppression. Thus, an NH2-terminal region of ~30 amino acids of Swi1 contains all the necessary information for in vivo prion formation, maintenance, and transmission. This PrD is unique in size and composition: glutamine free, asparagine rich, and the smallest defined to date. Our findings broaden our understanding of what features allow a protein region to serve as a PrD.

Original languageEnglish (US)
Article numbere00206-17
JournalMolecular and cellular biology
Volume37
Issue number20
DOIs
StatePublished - Oct 1 2017

Keywords

  • Amyloids
  • Epigenetic inheritance
  • Prion
  • Prion domain
  • Prion domain (PrD)
  • Prions
  • Protein aggregation
  • SWI/SNF
  • Saccharomyces cerevisiae
  • Swi1
  • Yeast
  • Yeasts

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Analysis of small critical regions of Swi1 conferring prion formation, maintenance, and transmission'. Together they form a unique fingerprint.

  • Cite this