TY - JOUR
T1 - Analysis of sperm chromosome complements before, during, and after chemotherapy
AU - Martin, Renée H.
AU - Ernst, Scott
AU - Rademaker, Alfred
AU - Barclay, Leona
AU - Ko, Evelyn
AU - Summers, Nancy
N1 - Funding Information:
The authors are grateful for the cooperation and dedication of the cancer patients who made this study possible. The secretarial skills of Debbie Bell are gratefully acknowledged. This research was supported by the Medical Research Council of Canada, the Alberta Heritage Fund for Medical Research, and the Alberta Children’s Hospital Research Foundation.
PY - 1999/1/15
Y1 - 1999/1/15
N2 - Sperm chromosomal abnormalities were assessed in testicular cancer patients before, during, and after BEP (bleomycin, etoposide, cisplatin) chemotherapy (CT). Multicolor fluorescence in situ hybridization (FISH) analysis was employed to detect aneuploidy for chromosomes 1, 12, X and Y, and diploidy. Sperm samples were cryopreserved and coded before analysis to facilitate 'blind' analysis. Complete results at all time points was available for only one patient. A total of 60,400 sperm were analyzed: 20,004 before CT, 20,005 during CT, and 20,391 after CT. There was a significant increase in the frequency of 24,XY sperm during (0.33%) and post-CT (0.34%) compared to pre-CT (0.14%). This study suggests that there may be a significantly increased risk of chromosomal abnormalities in sperm of CT patients during and immediately post-CT, similar to that shown in animal models.
AB - Sperm chromosomal abnormalities were assessed in testicular cancer patients before, during, and after BEP (bleomycin, etoposide, cisplatin) chemotherapy (CT). Multicolor fluorescence in situ hybridization (FISH) analysis was employed to detect aneuploidy for chromosomes 1, 12, X and Y, and diploidy. Sperm samples were cryopreserved and coded before analysis to facilitate 'blind' analysis. Complete results at all time points was available for only one patient. A total of 60,400 sperm were analyzed: 20,004 before CT, 20,005 during CT, and 20,391 after CT. There was a significant increase in the frequency of 24,XY sperm during (0.33%) and post-CT (0.34%) compared to pre-CT (0.14%). This study suggests that there may be a significantly increased risk of chromosomal abnormalities in sperm of CT patients during and immediately post-CT, similar to that shown in animal models.
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U2 - 10.1016/S0165-4608(98)00125-3
DO - 10.1016/S0165-4608(98)00125-3
M3 - Article
C2 - 9973940
AN - SCOPUS:0033555701
SN - 0165-4608
VL - 108
SP - 133
EP - 136
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
IS - 2
ER -
