Analysis of suborganellar fractions from spinach and pea chloroplasts for calmodulin-binding proteins

D. M. Roberts, R. E. Zielinski, M. Schleicher, D. M. Watterson

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Purified chloroplasts from spinach and pea leaves were subfractionated into envelope, thylakoid, and stroma fractions and were analyzed for calmodulin-binding proteins using a 125I-calmodulin gel overlay assay. Calmodulin binding was primarily associated with a major polypeptide (M(r) 33,000) in the envelope membrane fraction. In contrast, major calmodulin-binding proteins were not detected in the thylakoid or stroma fractions. Our results provide the first evidence of calmodulin-binding proteins in the chloroplast envelope, and raise the possibility that calmodulin may contribute to the regulation of chloroplast function through its interaction with calmodulin-binding proteins in the chloroplast envelope. In addition, our results combined with those of other investigators support the proposal that subcellular organelles may be a primary site of calmodulin action.

Original languageEnglish (US)
Pages (from-to)1644-1647
Number of pages4
JournalJournal of Cell Biology
Volume97
Issue number5 I
DOIs
StatePublished - 1983

ASJC Scopus subject areas

  • Cell Biology

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