Abstract
Heterotrimeric G protein-coupled receptors can activate the mitogen- activated protein kinase (MAPK) cascade. Recent studies using pharmacological inhibitors or dominant-negative mutants of signaling molecules have advanced our understanding of the pathways from G protein-coupled receptors to MAPK. However, molecular genetic analysis of these pathways is inadequate in mammalian cells. Here, using the well characterized G(s)(α-) and protein kinase A-deficient S49 mouse lymphoma cells, we provide the molecular genetic evidence that G(s)α is responsible for transducing the β-adrenergic receptor signal to MAPK in a protein kinase A-dependent pathway involving Rap1 and Raf (but not Ras) molecules.
Original language | English (US) |
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Pages (from-to) | 14533-14537 |
Number of pages | 5 |
Journal | Journal of Biological Chemistry |
Volume | 273 |
Issue number | 23 |
DOIs | |
State | Published - Jun 5 1998 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology