Analysis of the G(s)/mitogen-activated protein kinase pathway in mutant S49 cells

Yong Wan, Xin Yun Huang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

Heterotrimeric G protein-coupled receptors can activate the mitogen- activated protein kinase (MAPK) cascade. Recent studies using pharmacological inhibitors or dominant-negative mutants of signaling molecules have advanced our understanding of the pathways from G protein-coupled receptors to MAPK. However, molecular genetic analysis of these pathways is inadequate in mammalian cells. Here, using the well characterized G(s)(α-) and protein kinase A-deficient S49 mouse lymphoma cells, we provide the molecular genetic evidence that G(s)α is responsible for transducing the β-adrenergic receptor signal to MAPK in a protein kinase A-dependent pathway involving Rap1 and Raf (but not Ras) molecules.

Original languageEnglish (US)
Pages (from-to)14533-14537
Number of pages5
JournalJournal of Biological Chemistry
Volume273
Issue number23
DOIs
StatePublished - Jun 5 1998

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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