Analysis on the types of chemokines expressed by the murine thymic epithelial cell line MTEC1

L. Xie; X. Qian; S. Gong; W. Chen

doi:10.1007/BF02887182

Analysis on the types of chemokines expressed by the murine thymic epithelial cell line MTEC1

L. Xie, X. Qian, S. Gong, W. Chen

Pathology

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5 Scopus citations

Abstract

Six kinds of chemokines including SDF-1α, IP-10, KC, RANTES, MCP-1 and Lymphotactin have been amplified using the primers designed by ourselves with RT-PCR from the total RNA of a medullary-type murine thymic epithelial cell line MTEC1 cells. The amplified cDNA fragments of the chemokines have been identified by nucleotide sequencing and Northern blot analysis. The results demonstrate that there are two different splicing forms of SDF-1 mRNA existing in MTEC1. The concentrated culture supernatant of MTEC1 shows an intermediate level of chemotaxis to CD4^-CD8^- thymocytes, which conforms to the biological function of SDF-1.

Original language	English (US)
Pages (from-to)	1098-1101
Number of pages	4
Journal	Chinese Science Bulletin
Volume	45
Issue number	12
DOIs	https://doi.org/10.1007/BF02887182
State	Published - 2000

Keywords

Analysis
Chemokines
Thymic epithelial cells

ASJC Scopus subject areas

General

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10.1007/BF02887182

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title = "Analysis on the types of chemokines expressed by the murine thymic epithelial cell line MTEC1",

abstract = "Six kinds of chemokines including SDF-1α, IP-10, KC, RANTES, MCP-1 and Lymphotactin have been amplified using the primers designed by ourselves with RT-PCR from the total RNA of a medullary-type murine thymic epithelial cell line MTEC1 cells. The amplified cDNA fragments of the chemokines have been identified by nucleotide sequencing and Northern blot analysis. The results demonstrate that there are two different splicing forms of SDF-1 mRNA existing in MTEC1. The concentrated culture supernatant of MTEC1 shows an intermediate level of chemotaxis to CD4-CD8- thymocytes, which conforms to the biological function of SDF-1.",

keywords = "Analysis, Chemokines, Thymic epithelial cells",

author = "L. Xie and X. Qian and S. Gong and W. Chen",

note = "Funding Information: Acknowledgements This work was supported by the International Center for Genetic Engineering and Biotechnology (ICGEB) (Grant No. 981016) and China Postdoctoral Science Foundation.",

year = "2000",

doi = "10.1007/BF02887182",

language = "English (US)",

volume = "45",

pages = "1098--1101",

journal = "Chinese Science Bulletin",

issn = "1001-6538",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Analysis on the types of chemokines expressed by the murine thymic epithelial cell line MTEC1

AU - Xie, L.

AU - Qian, X.

AU - Gong, S.

AU - Chen, W.

N1 - Funding Information: Acknowledgements This work was supported by the International Center for Genetic Engineering and Biotechnology (ICGEB) (Grant No. 981016) and China Postdoctoral Science Foundation.

PY - 2000

Y1 - 2000

N2 - Six kinds of chemokines including SDF-1α, IP-10, KC, RANTES, MCP-1 and Lymphotactin have been amplified using the primers designed by ourselves with RT-PCR from the total RNA of a medullary-type murine thymic epithelial cell line MTEC1 cells. The amplified cDNA fragments of the chemokines have been identified by nucleotide sequencing and Northern blot analysis. The results demonstrate that there are two different splicing forms of SDF-1 mRNA existing in MTEC1. The concentrated culture supernatant of MTEC1 shows an intermediate level of chemotaxis to CD4-CD8- thymocytes, which conforms to the biological function of SDF-1.

AB - Six kinds of chemokines including SDF-1α, IP-10, KC, RANTES, MCP-1 and Lymphotactin have been amplified using the primers designed by ourselves with RT-PCR from the total RNA of a medullary-type murine thymic epithelial cell line MTEC1 cells. The amplified cDNA fragments of the chemokines have been identified by nucleotide sequencing and Northern blot analysis. The results demonstrate that there are two different splicing forms of SDF-1 mRNA existing in MTEC1. The concentrated culture supernatant of MTEC1 shows an intermediate level of chemotaxis to CD4-CD8- thymocytes, which conforms to the biological function of SDF-1.

KW - Analysis

KW - Chemokines

KW - Thymic epithelial cells

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JO - Chinese Science Bulletin

JF - Chinese Science Bulletin

IS - 12

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Analysis on the types of chemokines expressed by the murine thymic epithelial cell line MTEC1

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Keywords

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