Analytical and biological variability in biomarker measurement in the Hispanic Community Health Study/Study of Latinos

Bharat Thyagarajan*, Annie Green Howard, Ramon Durazo-Arvizu, John H. Eckfeldt, Marc D. Gellman, Ryung S. Kim, Kiang Liu, Armando J. Mendez, Frank J. Penedo, Gregory A. Talavera, Marston E. Youngblood, Lihui Zhao, Daniela Sotres-Alvarez

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


Background Biomarker variability, which includes within-individual variability (CVI), between-individual variability (CVG) and methodological variability (CVP + A) is an important determinant of our ability to detect biomarker-disease associations. Estimates of CVI and CVG may be population specific and little data exists on biomarker variability in diverse Hispanic populations. Hence, we evaluated all 3 components of biomarker variability in the Hispanic Community Health Study/Study of Latinos (HCHS/SOL) using repeat blood collections (n = 58) and duplicate blood measurements (n = 761–929 depending on the biomarker). Methods We estimated the index of individuality (II) ((CVI + CVP + A) / CVG) for 41 analytes and evaluated differences in the II across sexes and age groups. Results Biomarkers such as fasting glucose, triglycerides and ferritin had substantially higher inter-individual variability and lower II in HCHS/SOL as compared to the published literature. We also found significant sex-specific differences in the II for neutrophil count, platelet count, hemoglobin, % eosinophils and fasting glucose. The II for fasting insulin, post oral glucose tolerance test glucose and cystatin C was significantly higher among the 18–44 y age group as compared to the 45 + y age group. Conclusions The implications of these findings for determining biomarker-disease associations in Hispanic populations need to be evaluated in future studies.

Original languageEnglish (US)
Pages (from-to)129-137
Number of pages9
JournalClinica Chimica Acta
StatePublished - Dec 1 2016


  • Analytical variation
  • Biomarker variability
  • Hispanics

ASJC Scopus subject areas

  • Biochemistry, medical
  • Biochemistry
  • Clinical Biochemistry


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