Anatomical organization of the limb premotor network in the turtle (Chrysemys picta) revealed by in vitro transport of biocytin and neurobiotin

Ramin Sarrafizadeh*, James Charles Houk

*Corresponding author for this work

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The in vitro turtle brainstem‐cerebellum preparation has been a valuable tool in the study of central motor programs. In the present study, we investigate the anatomical organization of the turtle rubrocerebellar limb premotor network and its sensory connections in vitro by combining the rapid anterograde and retrograde transport of neurobiotin and biocytin with the extended viability of the isolated turtle brainstem‐cerebellum. These compounds retrogradely labeled soma, dendrites, and axons, and orthogradely labeled axons and, to a lesser extent, terminals. The chelonian red nucleus receives a dense input form the contralateral lateral cerebellar nucleus and projects heavily to the contralateral spinal cord. Rubral axons sparsley innervate the lateral cerebellar nucleus and project heavily to the lateral reticular nucleus. Lateral reticular axons heavily innervate the lateral cerebellar nucleus before terminating in the pars laterlalis of the cerebellar cortex as mossy fibers. These prominent, recurrent loops among the lateral cerebellar nucleus, red nucleus, and lateral reticular nucleus constitute the turtle rubrocerebellar limb premotor network. Sensory inputs to the red nucleus orginate in the contralateral dorsal column nuclei, the principle trigeminal nucleus, and the spinothalamic system. These sites project bilaterally to the lateral reticular nucleus. The lateral cerebellar nucleus receives a contralateral input from the dorsal column nuclei. The red nucleus projects sparsely to the dorsal column nuclei. The red nucleus also receives an ipsilateral descending projection from the suprapeduncular nucleus, located in the diencephalon, and an ascending input from the rostral rhombencephalic reticular formation. An ipsilateral descending pathway originating in the red nucleus is likely to be the rubro‐olivary tract. © 1994 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)137-159
Number of pages23
JournalJournal of Comparative Neurology
Volume344
Issue number1
DOIs
StatePublished - Jan 1 1994

Keywords

  • anterograde
  • lateral cerebellar nucleus
  • lateral reticular nucleus
  • red nucleus
  • retrograde

ASJC Scopus subject areas

  • Neuroscience(all)

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