Angiogenesis without functional outcome after mononuclear stem cell transplant in a doxorubicin-induced dilated myocardiopathy murine model

Katherine Athayde Teixeira Carvalho*, R. B. Simeoni, L. C. Guarita-Souza, J. C. Francisco, E. Abdelwahid, N. I. Myiague, J. C. Chachques, L. A. Rivetti, L. Oliveira, M. Malvezzi, M. Olandoski, W. Gremski

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Objectives: Cell transplantation is considered a novel approach in the treatment of myocardiopathy. The objective of this study was to evaluate the effects of autologous mononuclear stem cell therapy in doxorubicin-induced dilated myocardiopathy by conducting both functional and histopathologic analysis. Methods: Seventy male rats were doxorubicin injected intraperitoneally for 2 weeks. At 1 month, the animals that had demonstrated left ventricular ejection fractions less than 40% were randomly divided into a mononuclear stem cell group and controls. Mononuclear stem cells were isolated. All animals underwent echocardiographic study: baseline, pre-cell therapy, and at 1 month post-cell therapy, and analyzed by the nonparametric Mann-Whitney test. Transplants were performed by subepicardial injections. Standard staining was performed. Results: Twenty-three animals were randomly treated: mononuclear stem cell and control groups, with 11 rats completing the study. Cell viability was 85%. Mononuclear stem cells (n=5; 5×106 cells/300 μL medium) and control (n=6; 300 μL medium) were used. The resulting left ventricular ejection fraction in the cell therapy group was not significantly different compared with controls (p=0.54). New vessels were demonstrated in the subepicardial region. Conclusions: Autologous mononuclear stem cell therapy was not functionally effective in doxorubicin-induced dilated myocardiopathy in the animal model under study with the experimental conditions, despite occurrence of angiogenic activity.

Original languageEnglish (US)
Pages (from-to)431-438
Number of pages8
JournalInternational Journal of Artificial Organs
Volume31
Issue number5
DOIs
StatePublished - May 2008

Keywords

  • Bone marrow stem cells
  • Dilated myocardiopathy
  • Doxorubicin
  • Echocardiographic

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

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    Carvalho, K. A. T., Simeoni, R. B., Guarita-Souza, L. C., Francisco, J. C., Abdelwahid, E., Myiague, N. I., Chachques, J. C., Rivetti, L. A., Oliveira, L., Malvezzi, M., Olandoski, M., & Gremski, W. (2008). Angiogenesis without functional outcome after mononuclear stem cell transplant in a doxorubicin-induced dilated myocardiopathy murine model. International Journal of Artificial Organs, 31(5), 431-438. https://doi.org/10.1177/039139880803100509