ANP32A regulates histone H3 acetylation and promotes leukemogenesis

Xuejing Yang, Bin Lu, Xueqin Sun, Cuijuan Han, Chunling Fu, Kailin Xu, Min Wang, Dengju Li, Zhichao Chen, Puneet Opal, Qiang Wen, John D. Crispino, Qian Fei Wang, Zan Huang*

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Deregulation of key regulators of histone modification is important in the initiation and progression of human leukemia. Acidic leucine-rich nuclear phosphoprotein-32A (ANP32A) participates in histone acetylation and its role in acute myeloid leukemia remains unclear. Here we observed significant upregulation of ANP32A in primary AML cells, which was essential for AML cell proliferation, survival, and colony formation. Integrative analysis of the genome-wide histone H3 acetylation and gene expression demonstrated that ANP32A deficiency reduced histone H3 acetylation, in accordance with changes in gene expression. Notably, significant histone H3 acetylation enrichment was associated with mRNA changes in lipid-related genes, including APOC1, PCSK9, P2RX1, and LPPR3. Indeed, over-expression of APOC1 partially compensated the proliferation-defect phenotype in ANP32A deficient AML cells while APOC1 knockdown alone mimicked the effect of ANP32A deficiency. Collectively, our data indicate that ANP32A is a novel regulator of histone H3 acetylation and promotes leukemogenesis.

Original languageEnglish (US)
Pages (from-to)1587-1597
Number of pages11
JournalLeukemia
Volume32
Issue number7
DOIs
StatePublished - Jul 1 2018

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'ANP32A regulates histone H3 acetylation and promotes leukemogenesis'. Together they form a unique fingerprint.

  • Cite this

    Yang, X., Lu, B., Sun, X., Han, C., Fu, C., Xu, K., Wang, M., Li, D., Chen, Z., Opal, P., Wen, Q., Crispino, J. D., Wang, Q. F., & Huang, Z. (2018). ANP32A regulates histone H3 acetylation and promotes leukemogenesis. Leukemia, 32(7), 1587-1597. https://doi.org/10.1038/s41375-018-0010-7