Antagomir dependent microRNA-205 reduction enhances adhesion ability of human corneal epithelial keratinocytes

Jun Li, Hua Bai, Yong Zhu, Xiao Yan Wang, Fang Wang, Jun Wu Zhang, Robert M. Lavker, Jia Yu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Objective: To investigate the effect of microRNA-205 reduction by antagomirs on adhesion ability of normal human corneal epithelial keratinocytes (NHCEKs). Methods: Antagomir-205, complementary and inhibitory to microRNA-205, was used to suppress endogenous microRNA-205 in NHCEKs. The adhesion ability of treated NHCEKs was then assessed by cell adhesion assay. Immunoblot and immunohistochemistry were conducted to determine the level of two focal adhesion-related proteins, focal adhesion kinase (FAK) and paxillin (Pax). Phalloidin staining was performed to measure the level of filamentous actin in antagomir-treated NHCEKs. Results: Antagomir-205 markedly reduced the level of microRNA-205 in NHCEKs and significantly enhanced adhesion ability of NHCEKs (P<0.01). Further protein analysis validated that inhibition of microRNA-205 increased the number of phosphorylated FAK and phosphorylated Pax, and decreased filamentous actin. Conclusion: Our findings suggest that microRNA-205 has down-regulating effect on cell motility in NHCEKs.

Original languageEnglish (US)
Pages (from-to)65-70
Number of pages6
JournalChinese Medical Sciences Journal
Volume25
Issue number2
DOIs
StatePublished - Jun 2010

Keywords

  • Cell signaling
  • Corneal epithelium
  • MicroRNA

ASJC Scopus subject areas

  • Medicine(all)

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