Anti-CD11b monoclonal antibody improves myocardial function after six hours of hypothermic storage

Joseph M. Forbess; Takeshi Hiramatsu; Fumikazu Nomura; Takuya Miura; G. King Farrington; Karen Sokolowski; Mark Bree; John E. Mayer

doi:10.1016/0003-4975(95)00618-U

Anti-CD11b monoclonal antibody improves myocardial function after six hours of hypothermic storage

Joseph M. Forbess, Takeshi Hiramatsu, Fumikazu Nomura, Takuya Miura, G. King Farrington, Karen Sokolowski, Mark Bree, John E. Mayer^*

^*Corresponding author for this work

Surgery, Cardiac Surgery Division

Research output: Contribution to journal › Article › peer-review

Abstract

Background.: The shortage of pediatric heart donors often necessitates considerable travel time and, as a result, prolonged donor heart ischemia. This excessive hypothermic storage may contribute markedly to myocardial dysfunction in the recipient. Methods.: We investigated the role of leukocyte-endothelial interactions in this dysfunction in an isolated, immature (mean age, 11.8 ± 1.6 days) swine heart model using a monoclonal antibody against a leukocyte adhesion molecule. We studied a total of 20 hearts subjected to 6 hours of cardioplegic arrest at 4°C. Group M1/70 (n = 6) received at reperfusion 15 μg/mL of a monoclonal antibody F(ab′)₂ fragment to CD11b, the α-subunit of the leukocyte adhesion molecule Mac-1. Group MB10.6 (n = 8) received 15 μg/mL of the swine unreactive F(ab′)₂ MB10.6, and the third group received saline vehicle. Results.: Administration of M1/70 resulted in improved postischemic recovery of ventricular function compared with the two control groups (p < 0.05). Conclusions.: These data implicate leukocyte-endothelial interactions mediated by the leukocyte adhesion molecule CD11b in myocardial dysfunction after long-term hypothermic ischemia. Specific antiadhesion strategies such as this may safely extend storage time for pediatric donor hearts.

Original language	English (US)
Pages (from-to)	1238-1244
Number of pages	7
Journal	The Annals of thoracic surgery
Volume	60
Issue number	5
DOIs	https://doi.org/10.1016/0003-4975(95)00618-U
State	Published - Nov 1995

ASJC Scopus subject areas

Surgery
Pulmonary and Respiratory Medicine
Cardiology and Cardiovascular Medicine

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title = "Anti-CD11b monoclonal antibody improves myocardial function after six hours of hypothermic storage",

abstract = "Background.: The shortage of pediatric heart donors often necessitates considerable travel time and, as a result, prolonged donor heart ischemia. This excessive hypothermic storage may contribute markedly to myocardial dysfunction in the recipient. Methods.: We investigated the role of leukocyte-endothelial interactions in this dysfunction in an isolated, immature (mean age, 11.8 ± 1.6 days) swine heart model using a monoclonal antibody against a leukocyte adhesion molecule. We studied a total of 20 hearts subjected to 6 hours of cardioplegic arrest at 4°C. Group M1/70 (n = 6) received at reperfusion 15 μg/mL of a monoclonal antibody F(ab′)2 fragment to CD11b, the α-subunit of the leukocyte adhesion molecule Mac-1. Group MB10.6 (n = 8) received 15 μg/mL of the swine unreactive F(ab′)2 MB10.6, and the third group received saline vehicle. Results.: Administration of M1/70 resulted in improved postischemic recovery of ventricular function compared with the two control groups (p < 0.05). Conclusions.: These data implicate leukocyte-endothelial interactions mediated by the leukocyte adhesion molecule CD11b in myocardial dysfunction after long-term hypothermic ischemia. Specific antiadhesion strategies such as this may safely extend storage time for pediatric donor hearts.",

author = "Forbess, {Joseph M.} and Takeshi Hiramatsu and Fumikazu Nomura and Takuya Miura and Farrington, {G. King} and Karen Sokolowski and Mark Bree and Mayer, {John E.}",

year = "1995",

month = nov,

doi = "10.1016/0003-4975(95)00618-U",

language = "English (US)",

volume = "60",

pages = "1238--1244",

journal = "Annals of Thoracic Surgery",

issn = "0003-4975",

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T1 - Anti-CD11b monoclonal antibody improves myocardial function after six hours of hypothermic storage

AU - Forbess, Joseph M.

AU - Hiramatsu, Takeshi

AU - Nomura, Fumikazu

AU - Miura, Takuya

AU - Farrington, G. King

AU - Sokolowski, Karen

AU - Bree, Mark

AU - Mayer, John E.

PY - 1995/11

Y1 - 1995/11

N2 - Background.: The shortage of pediatric heart donors often necessitates considerable travel time and, as a result, prolonged donor heart ischemia. This excessive hypothermic storage may contribute markedly to myocardial dysfunction in the recipient. Methods.: We investigated the role of leukocyte-endothelial interactions in this dysfunction in an isolated, immature (mean age, 11.8 ± 1.6 days) swine heart model using a monoclonal antibody against a leukocyte adhesion molecule. We studied a total of 20 hearts subjected to 6 hours of cardioplegic arrest at 4°C. Group M1/70 (n = 6) received at reperfusion 15 μg/mL of a monoclonal antibody F(ab′)2 fragment to CD11b, the α-subunit of the leukocyte adhesion molecule Mac-1. Group MB10.6 (n = 8) received 15 μg/mL of the swine unreactive F(ab′)2 MB10.6, and the third group received saline vehicle. Results.: Administration of M1/70 resulted in improved postischemic recovery of ventricular function compared with the two control groups (p < 0.05). Conclusions.: These data implicate leukocyte-endothelial interactions mediated by the leukocyte adhesion molecule CD11b in myocardial dysfunction after long-term hypothermic ischemia. Specific antiadhesion strategies such as this may safely extend storage time for pediatric donor hearts.

AB - Background.: The shortage of pediatric heart donors often necessitates considerable travel time and, as a result, prolonged donor heart ischemia. This excessive hypothermic storage may contribute markedly to myocardial dysfunction in the recipient. Methods.: We investigated the role of leukocyte-endothelial interactions in this dysfunction in an isolated, immature (mean age, 11.8 ± 1.6 days) swine heart model using a monoclonal antibody against a leukocyte adhesion molecule. We studied a total of 20 hearts subjected to 6 hours of cardioplegic arrest at 4°C. Group M1/70 (n = 6) received at reperfusion 15 μg/mL of a monoclonal antibody F(ab′)2 fragment to CD11b, the α-subunit of the leukocyte adhesion molecule Mac-1. Group MB10.6 (n = 8) received 15 μg/mL of the swine unreactive F(ab′)2 MB10.6, and the third group received saline vehicle. Results.: Administration of M1/70 resulted in improved postischemic recovery of ventricular function compared with the two control groups (p < 0.05). Conclusions.: These data implicate leukocyte-endothelial interactions mediated by the leukocyte adhesion molecule CD11b in myocardial dysfunction after long-term hypothermic ischemia. Specific antiadhesion strategies such as this may safely extend storage time for pediatric donor hearts.

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Anti-CD11b monoclonal antibody improves myocardial function after six hours of hypothermic storage

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